Anti-Sarm1 Monoclonal Antibody for WB, ELISA - Q6PDS3

Core Product Specifications and Parameters

Biological Background: Sarm1 Function and Localization

• SARM1 (Sterile alpha and TIR motif-containing protein 1), also known as NAD(+) hydrolase SARM1, is encoded by the Sarm1 gene (Kiaa0524) and undergoes alternative splicing. The protein functions primarily as an NAD(+) hydrolase, catalyzing the cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR), and nicotinamide, thereby regulating NAD(+) metabolism and playing a pivotal role in axonal degeneration following injury. Related references: PMID:25818290 PMID:26686637 PMID:27735788
• It acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor (TLR) signaling by promoting Wallerian degeneration—an injury-induced programmed subcellular death involving the degeneration of the axon distal to the injury site. Related references: PMID:21555464 PMID:22678360 PMID:25818290
• Upon injury, SARM1 is activated and its NAD(+) cleavage activity triggers NAD(+) depletion, leading to cytoskeletal degradation and axon destruction. Related references: PMID:28334607
• SARM1 can activate neuronal cell death in response to stress and regulates dendritic arborization through the MAPK4-JNK pathway. Related references: PMID:19587044 PMID:17724133 PMID:21555464
• In innate immunity, SARM1 inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38. Related references: PMID:21555464
• It also acts as a non-self dsDNA sensor in a sequence-independent manner to elicit NAD(+) degradation and promote programmed cell death (By similarity). SARM1 is able to hydrolyze NADP(+) but not other NAD(+)-related molecules (By similarity).
• SARM1 localizes to the cytoplasm, cell projections (including axons and dendrites), synapses, and mitochondria. It is widely expressed in the brain and neurons, including photoreceptor cells of the neural retina. Related references: PMID:21555464 PMID:32312889
• The protein is subject to phosphorylation and alternative splicing, and is annotated with keywords such as Hydrolase, Immunity, Innate immunity, Neurogenesis, and Mitochondrion.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1-100 of mouse Sarm1; the antibody likely targets the N-terminal region. This should be considered when designing experiments or interpreting results.
• This mouse monoclonal antibody is suitable for WB and ELISA. For Western blot, consider optimizing antibody dilution and blocking conditions for your specific sample type. The predicted protein weight is approximately 79 kDa, but confirm migration in your experimental system.
• Cross-reactivity has been validated for human, mouse, and rat samples. When using other species, validate binding specificity and signal intensity.
• For ELISA, use appropriate antigen coating and detection protocols; perform a titration to determine optimal antibody concentration.
• As SARM1 is involved in axonal degeneration and innate immunity, the antibody can be used to study neurodegenerative processes, stress responses, and immune signaling pathways.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, providing diagnostic manufacturers and research institutes with comprehensive access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials. With this anti-Sarm1 antibody, researchers can effectively investigate axonal degeneration and innate immune pathways. Our expert supply chain and technical support ensure reliable procurement from concept to clinic.