Anti-SARDH Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UL12

Core Product Specifications and Parameters

Biological Background: SARDH Function and Localization

• SARDH (sarcosine dehydrogenase) is a mitochondrial enzyme that catalyzes the final step in the oxidative degradation of choline to glycine by converting sarcosine into glycine.
• Alternative names include BPR-2, with gene symbol SARDH (also known as DMGDHL1).
• The protein localizes to the mitochondrial matrix, consistent with its role in choline metabolism.
• SARDH is predominantly expressed in pancreas, liver, and kidney, reflecting its tissue-specific metabolic function.
• It is a flavoprotein dependent on FAD, classified as an oxidoreductase.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity.
• Alternative splicing generates multiple isoforms, and genetic variants in SARDH have been associated with disease.
• The protein contains a transit peptide for mitochondrial import and has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 300–560 of human SARDH, a region that is likely conserved across species given the reported mouse and rat cross-reactivity.
• For Western blot (WB), the predicted molecular weight is ~101 kDa; use appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• Endogenous SARDH expression is highest in liver, pancreas, and kidney; consider using tissue lysates from these sources as positive controls.
• In immunofluorescence (IF/ICC), SARDH is expected to localize to mitochondria; co-staining with a mitochondrial marker can help confirm specificity.
• For ELISA applications, the polyclonal nature may provide robust signal, but titration of both antibody and antigen is recommended to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of high-quality IVD raw materials, including optimized polyclonal antibodies like this anti-SARDH antibody. Our one-stop platform streamlines procurement of validated antibodies, bulk reagents, and ancillary supplies for choline metabolism and mitochondrial research applications. Contact us to explore custom sourcing and technical consultation services.