Anti-SARA/ZFYVE9 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O95405

Core Product Specifications and Parameters

Biological Background: SARA/ZFYVE9 Function and Localization

• ZFYVE9 (SARA) is a zinc finger FYVE domain-containing protein that acts as an adaptor linking SMAD2/SMAD3 to the TGF-beta receptor, facilitating SMAD phosphorylation and signal transduction.
• The FYVE domain targets the protein to phosphatidylinositol 3-phosphate (PtdIns3P)-enriched membranes of early endosomes, where it recruits SMAD2/3 and the receptor complex.
• SARA interacts with SMAD2 and SMAD3 via its SMAD-binding domain (SBD), and this interaction is critical for TGF-beta-mediated transcriptional regulation.
• It is ubiquitously expressed, with notable presence in cerebrovascular smooth muscle cells and reactive astrocytes in the brain.
• The protein contains a zinc-finger FYVE domain that coordinates two zinc ions, essential for endosomal membrane association.
• SARA undergoes phosphorylation, although the functional significance is not fully elucidated.
• Alternative splicing generates multiple isoforms, potentially modulating protein interactions and signaling outcomes.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-100) of human SARA; antibody reactivity likely targets this portion, which includes sequences upstream of the FYVE domain.
• For Western blotting, a band at ~156 kDa is expected; consider using appropriate molecular weight markers and lysates from TGF-beta-stimulated cells to enhance detection.
• In IHC-P, antigen retrieval may be required; validate staining in known positive tissues such as placenta or brain samples.
• ELISA protocols may require optimization of coating antigen and antibody dilution; recombinant SARA protein can serve as a control.
• Cross-reactivity with mouse and rat is predicted based on sequence homology; confirm in relevant sample systems for species-specific studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated access to validated IVD raw materials, including this SARA rabbit monoclonal antibody, optimized for TGF-beta signaling research. Our portfolio supports diagnostic manufacturers with bulk ancillary reagents, matched antibody pairs, and custom sourcing of rare target proteins. From early development to clinical application, CamelBio streamlines your supply chain.