Anti-SAR1B Rabbit Polyclonal Antibody for WB, ELISA - Q9Y6B6

Core Product Specifications and Parameters

Biological Background: SAR1B Function and Localization

• SAR1B (Secretion-associated Ras-related GTPase 1B) is a small GTPase of the Arf family that cycles between an active GTP-bound and inactive GDP-bound state.
• Active GTP-bound SAR1B inserts into the endoplasmic reticulum (ER) membrane and recruits the COPII coat complex to mediate vesicle budding for ER-to-Golgi transport.
  Related references: PMID:23433038, PMID:32358066, PMID:33186557
• SAR1B specifically interacts with the cargo receptor SURF4 to mediate the transport of lipid-carrying lipoproteins such as APOB and APOA1 from the ER to the Golgi, thereby regulating lipid homeostasis.
  Related references: PMID:32358066, PMID:33186557
• In addition to its vesicle trafficking role, SAR1B acts as a leucine sensor: in the absence of leucine, it binds the GATOR2 complex via MIOS to inhibit TORC1 signaling, a function independent of its GTPase activity.
  Related references: PMID:34290409
• Subcellular localization includes the endoplasmic reticulum membrane, Golgi stack membrane, cytosol, and lysosome membrane.
• Expressed in multiple tissues, notably the small intestine, liver, muscle, and brain.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein spanning amino acids 1-198 of human SAR1B; it may recognize epitopes within the N-terminal GTPase domain.
• Validated for Western blot (WB) and ELISA applications. For WB, the predicted molecular weight is ~22 kDa; validate in human cell lines or tissues expressing SAR1B.
• Cross-reactivity has been confirmed with human SAR1B; reactivity with other species has not been tested. Consider optimizing conditions for your specific sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic developments from concept to clinical application. This anti-SAR1B polyclonal antibody aids research into ER-to-Golgi trafficking, lipid metabolism, and related disorders. In addition to antibodies, we offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to streamline your IVD assay development.