Anti-SAP130 Rabbit Polyclonal Antibody for WB, ELISA - Q9H0E3

Core Product Specifications and Parameters

Biological Background: SAP130 Function and Localization

• SAP130, also known as 130 kDa Sin3-associated polypeptide or Sin3-associated polypeptide p130, is a subunit of the histone deacetylase (HDAC) complex.
• It functions as a transcriptional repressor, potentially mediating interactions between the mSin3A corepressor complex and other regulatory complexes.
• Localizes predominantly to the nucleus, consistent with its role in transcription regulation.
• Subject to multiple post-translational modifications including acetylation, methylation, phosphorylation, and ubiquitination (isopeptide bond formation), indicating dynamic regulation.
• Expressed in various cancer cell lines, suggesting potential relevance in cancer biology.
• Undergoes alternative splicing, which may generate isoforms with distinct functions.
• Part of the human reference proteome, ensuring broad research applicability.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment spanning amino acids 819–1048, located in the C-terminal region of human SAP130; ensure that your target epitope is preserved in your samples.
• For Western blot (WB), consider using whole-cell lysates from human or mouse cell lines, as expression has been reported in cancer cell lines and cross-reactivity with mouse is documented.
• Due to the polyclonal nature, batch-to-batch consistency for ELISA should be validated with appropriate controls.
• Expression levels may vary among tissues and cell lines; validate the antibody in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials. This anti-SAP130 polyclonal antibody, targeting a key component of the HDAC transcriptional repression complex, is part of our extensive collection for epigenetics and cancer research. Beyond individual reagents, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your diagnostic development from concept to clinic.