Anti-SAMM50 Monoclonal Antibody for WB, IHC-P, ELISA - Q9Y512

Core Product Specifications and Parameters

Biological Background: SAMM50 Function and Localization

• SAMM50 (Sorting and assembly machinery component 50 homolog), aliases TRG-3/SAM50/OMP85/TOB55, is a mitochondrial outer membrane protein critical for organelle integrity and respiratory function.
• It maintains mitochondrial cristae structure and ensures proper assembly of the respiratory chain complexes (PubMed:22252321, PubMed:25781180). Related references: PMID:22252321 PMID:25781180
• SAMM50 is required for the integration of TOMM40 into the TOM complex, a key step in mitochondrial protein import (PubMed:15644312). Related references: PMID:15644312
• The protein localizes primarily to the mitochondrial outer membrane but has also been detected in the cytoplasm and other mitochondrial subcompartments.
• It is a transmembrane beta-barrel protein subject to methylation, hinting at regulatory post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen spans residues 300-400, likely generating an antibody that recognizes a linear epitope in the C-terminal region; it may be suitable for applications requiring denatured targets.
• For WB, use standard reducing/denaturing conditions; a band near 52 kDa is expected.
• In IHC-P, optimize antigen retrieval and antibody titration; cross-reactivity with mouse and rat tissues has been observed.
• ELISA performance should be validated with recombinant or native SAMM50 in the relevant buffer system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner, providing one-stop access to IVD raw materials including validated antibodies, assay-ready antibody pairs, and ancillary reagents. This SAMM50 antibody supports mitochondrial and metabolic disease research, and our supply network can source rare-target reagents for your diagnostic development. Connect with our team to streamline your procurement from concept to clinic.