Anti-SAMHD1 Monoclonal Antibody for WB, IHC-P, ELISA - Q9Y3Z3

Core Product Specifications and Parameters

Biological Background: SAMHD1 Function and Localization

• SAMHD1 (SAM domain and HD domain-containing protein 1) is also known as DCIP, CHBL2, HDDC1, MOP-5, SBBI88, and hSAMHD1. It is a deoxynucleoside triphosphate triphosphohydrolase with key roles in antiviral restriction and DNA repair.
• SAMHD1 functions as a host restriction factor by depleting cellular dNTP pools via its dNTPase activity, thereby blocking reverse transcription of retroviruses such as HIV-1 in dendritic and myeloid cells (PubMed:19525956, PubMed:21613998, PubMed:21720370). Related references: PMID:19525956 PMID:21613998 PMID:21720370
• Beyond retroviruses, SAMHD1 restricts LINE-1 retrotransposons, linking it to genome stability maintenance (PubMed:24035396, PubMed:24217394, PubMed:29610582). Related references: PMID:24035396 PMID:24217394 PMID:29610582
• Phosphorylation at Thr-592 acts as a molecular switch: it inhibits dNTPase activity and antiviral function, while promoting DNA end resection at stalled replication forks via MRE11 activation (PubMed:23601106, PubMed:23602554, PubMed:29670289). Related references: PMID:23601106 PMID:23602554 PMID:29670289
• SAMHD1 localizes predominantly to the nucleus and associates with chromosomes, consistent with its role in DNA repair and replication fork processing.
• It is widely expressed in tissues including heart, skeletal muscle, spleen, liver, small intestine, placenta, lung, and peripheral blood leukocytes, but absent in brain and thymus (PubMed:11064105). Related references: PMID:11064105
• Post-translational modifications include acetylation, phosphorylation, and isopeptide bond formation; mutations in SAMHD1 are linked to Aicardi-Goutières syndrome, a type I interferonopathy.
• SAMHD1 also enhances immunoglobulin hypermutation in B-lymphocytes by promoting transversion mutation (By similarity).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 527-626 of human SAMHD1, a region containing the regulatory phosphorylation site Thr-592. Users should consider the phosphorylation status of SAMHD1 in their experimental system, as it may influence antibody binding; validation in relevant cell types or under specific stimuli is recommended.
• For Western blot (WB), the predicted molecular weight is 72 kDa. Use appropriate denaturing conditions and include positive controls (e.g., THP-1, HeLa, or spleen lysates) to confirm band specificity.
• For immunohistochemistry-paraffin (IHC-P), antigen retrieval (heat-mediated or enzymatic) is likely necessary. Test on known SAMHD1-expressing tissues (e.g., spleen, liver) and include isotype controls.
• For ELISA, the antibody can be used as capture or detection reagent; proper pairing and concentration optimization are advised. Perform cross-validation with the intended antigen source.
• Given SAMHD1's roles in antiviral defense and DNA damage response, researchers may assess its expression upon interferon stimulation or replication stress.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This SAMHD1 antibody supports research in antiviral immunity, DNA repair, and oncology; CamelBio can further supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your diagnostic development pipeline.