Anti-SAG (S-Arrestin) Polyclonal Antibody - P10523

Core Product Specifications and Parameters

Biological Background: S-Arrestin Function and Localization

• S-arrestin (also known as 48 kDa protein, retinal S-antigen, rod photoreceptor arrestin) is encoded by the SAG gene in humans.

• It binds to photoactivated, phosphorylated rhodopsin (RHO) and terminates RHO signaling by competing with transducin for the same binding site on RHO.

• This function is critical for preventing light-dependent degeneration of retinal photoreceptor cells. Related references: PMID:9565049

• S-arrestin is a member of the arrestin family, which regulates G protein-coupled receptor (GPCR) signaling.

• Subcellularly, it localizes to the photoreceptor outer segment, specifically to the proximal portion of rod outer segments, and is associated with the cell membrane and cilium.

• The protein is phosphorylated (phosphoprotein) and has a molecular weight of approximately 45 kDa.

• Tissue expression is predominantly in the retina, especially in rod photoreceptor cells.

• Mutations in SAG are associated with ocular diseases such as congenital stationary night blindness and retinitis pigmentosa.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein spanning amino acids 1–405 of human SAG (full-length), which may recognize the native protein across multiple applications.

• For Western blotting (WB), an expected band around 45 kDa should be observed; consider using retinal or photoreceptor-derived samples as positive control.

• In immunohistochemistry (IHC-P), SAG expression is predominantly in the retina; validate with appropriate tissue sections.

• The antibody cross-reacts with mouse and rat SAG, enabling its use in rodent models; however, confirm reactivity experimentally.

• For ELISA, the antibody may detect recombinant or native S-antigen; optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, bulk reagents, and custom sourcing solutions. This anti-SAG (S-arrestin) polyclonal antibody supports research in ophthalmology and GPCR signaling. Our team assists with technical selection and supply chain streamlining for diagnostic development.