Anti-SAFB2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q14151

Core Product Specifications and Parameters

Biological Background: SAFB2 Function and Localization

• SAFB2 (Scaffold attachment factor B2) is a DNA-binding protein that specifically binds to scaffold/matrix attachment regions (S/MARs), playing a role in chromatin organization and gene regulation.
• It functions as a transcriptional corepressor of the estrogen receptor, thereby modulating hormone-dependent transcription, and has been shown to inhibit cell proliferation.
• The protein localizes primarily to the nucleus but is also detected in the cytoplasm, consistent with its roles in transcription and RNA metabolism.
• Expression is highest in the central nervous system, with lower levels in the liver, and it is frequently detected in breast cancer cell lines, suggesting a role in tumor biology.
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitination, which may regulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-255) of human SAFB2; consider verifying antibody specificity using recombinant protein fragments or peptide competition assays in relevant sample systems.
• For Western blot, a band around 107 kDa is expected; optimize using whole lysates from human cell lines (e.g., HeLa, HEK293) or tissues expressing SAFB2. Mouse and rat orthologs may also be detected due to cross-reactivity.
• For immunohistochemistry (IHC-P), validate the antibody on formalin-fixed paraffin-embedded tissue sections with known SAFB2 expression, such as breast carcinoma or brain tissue, and include appropriate negative controls.
• In ELISA, use the recombinant immunogen as a positive control to establish assay parameters; note that polyclonal preparations may exhibit lot-to-lot variability.

CamelBio: Your One-Stop Sourcing Bridge

As a leading IVD raw-material partner, CamelBio provides comprehensive access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents—covering targets like SAFB2 that are pivotal in transcription regulation and cancer research. We source rare targets and custom preparations to bridge the gap from concept to clinical application, supporting diagnostic manufacturers and research laboratories with reliable, scalable supply solutions.