Anti-SAFB2 Rabbit Polyclonal Antibody for WB, ELISA - Q14151

Core Product Specifications and Parameters

Biological Background: SAFB2 Function and Localization

• SAFB2 (scaffold attachment factor B2) is a nuclear matrix protein that binds to scaffold/matrix attachment region (S/MAR) DNA, contributing to chromatin organization and nuclear architecture.
• It acts as an estrogen receptor corepressor, modulating transcriptional activity dependent on estrogen signaling.
• SAFB2 can inhibit cell proliferation, implicating a role in cell growth control and potentially tumor suppression.
• The protein localizes to both the nucleus and cytoplasm, indicating dynamic shuttling or multifaceted functions.
• High expression is observed in the central nervous system, with lower levels in the liver; it is also expressed in numerous breast cancer cell lines.
• Post-translational modifications include acetylation, methylation, phosphorylation, isopeptide bond formation, and ubiquitin conjugation, which may regulate its activity and stability.
• As a member of the SAFB protein family, SAFB2 contains RNA-binding motifs, linking it to post-transcriptional regulation.
• Alternative splicing yields multiple isoforms, potentially diversifying its functional repertoire.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-255) of human SAFB2 (NP_055464.1). When performing Western blotting, expect a band near 107 kDa; consider using nuclear-enriched extracts to enhance detection.
• Cross-reactivity with mouse and rat SAFB2 suggests suitability for rodent model studies. Validate antibody performance in your specific tissue or cell line of interest.
• For ELISA, optimize the coating antigen and antibody dilution under your assay conditions. In-house testing using recombinant SAFB2 protein or cell lysates is recommended.
• SAFB2 is a known binding partner of the nuclear matrix; harsh extraction methods (e.g., high salt, detergents) may be required to release the protein for immunoprecipitation or other applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-SAFB2 antibody supports investigation into chromatin biology, transcriptional regulation, and cancer cell signaling. Beyond research reagents, we offer validated antibody pairs, bulk ancillary materials, and tailored sourcing for rare targets, empowering your IVD development and manufacturing workflows.