Anti-SAFB Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q15424

Core Product Specifications and Parameters

Biological Background: SAFB Function and Localization

• SAFB (Scaffold attachment factor B1), also known as HSP27 ERE-TATA-binding protein, HAP, HET, or SAFB1, is an alternative-spliced nuclear protein that functions as an estrogen receptor corepressor and DNA/RNA-binding factor.
• It binds to scaffold/matrix attachment region (S/MAR) DNA and assembles a 'transcriptosomal' complex containing SR proteins and RNA polymerase II, coupling transcription and RNA processing (PubMed:9671816). Related references: PMID:9671816
• SAFB acts as an estrogen receptor corepressor and can bind to the HSP27 promoter to decrease its transcription, thereby negatively regulating cell proliferation (PubMed:12660241). Related references: PMID:12660241
• When associated with RBMX, SAFB binds and stimulates transcription from the SREBF1 promoter (By similarity).
• Subcellular localization is nuclear.
• It is ubiquitously expressed, with high levels in the central nervous system and low levels in the liver; also expressed in many breast cancer cell lines.
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitin conjugation (isopeptide bond), as indicated by keywords.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning the C‑terminal region (amino acids 815‑915) of human SAFB. This region may contribute to antibody specificity; cross‑reactivity with SAFB isoforms or related proteins should be evaluated in the relevant sample system.
• For Western blot, SAFB has a predicted molecular weight of ~103 kDa; consider using a low‑percentage (e.g., 8%) SDS‑PAGE gel and appropriate transfer conditions for high‑molecular‑weight proteins.
• For immunohistochemistry (IHC‑P), optimal antigen retrieval and detection conditions may vary. Validate the antibody in known positive tissues such as human CNS sections or breast cancer cell lines.
• In ELISA applications, determine optimal coating antigen and antibody dilutions empirically.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio is your trusted one‑stop partner for IVD raw‑material procurement, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Our SAFB rabbit monoclonal antibody supports research into transcription regulation, estrogen receptor corepression, and chromatin biology. We help diagnostic manufacturers and labs accelerate development from concept to clinic with comprehensive raw‑material solutions.