Anti-SAAL1 Polyclonal Antibody for WB, ELISA - Q96ER3

Core Product Specifications and Parameters

Biological Background: SAAL1 Function and Localization

• Protein SAAL1, also known as synoviocyte proliferation-associated in collagen-induced arthritis protein 1 (SPACIA1), is encoded by the SAAL1 gene in humans.
• SAAL1 plays a role in promoting the proliferation of synovial fibroblasts in response to pro-inflammatory stimuli, linking it to inflammatory joint diseases.
• The protein localizes predominantly to the nucleus, consistent with a potential regulatory function in cellular proliferation.
• At the protein level, SAAL1 is highly expressed in testis and ovary, with lower expression detected in lung, spleen, and heart.
• SAAL1 is classified as a phosphoprotein, suggesting its activity or interactions may be modulated by post-translational phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 175–474 of human SAAL1, providing a broad antigenic region for polyclonal antibody generation.
• For Western blotting, expect a specific band near 54 kDa; validate in a relevant positive control such as testis or ovary lysate.
• Confirmed cross-reactivity with mouse and rat allows for potential use in rodent models; however, verify performance in your sample system.
• For ELISA applications, consider using recombinant SAAL1 protein as a standard and confirm specificity against full-length target.
• Optimize antibody dilution and detection conditions experimentally; start with a recommended range (e.g., 1:500–1:2000 for WB) and adjust.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement pathway for IVD raw materials, including validated antibodies, antigen reagents, and ancillary supplies. This anti-SAAL1 polyclonal antibody supports research into inflammatory arthritis and synovial pathologies, and our team can assist with sourcing rare-target materials, optimized antibody pairs, and technical consultation to advance your diagnostic projects from concept to clinic.