Anti-SAA1 Polyclonal Antibody for WB and ELISA - P0DJI8

Core Product Specifications and Parameters

Biological Background: SAA1 Function and Localization

• SAA1 (Serum Amyloid A1) is a major acute phase protein whose expression is dramatically upregulated during inflammation. It is primarily synthesized by the liver and secreted into the plasma.
• The protein is produced as a preprotein with an N-terminal signal peptide, which is cleaved to yield the mature secreted form of approximately 12 kDa.
• SAA1 circulates in the blood predominantly associated with high-density lipoprotein (HDL) and can also bind heparin, influencing lipid metabolism and immune responses.
• Post-translational methylation has been identified on SAA1, which may regulate its stability or function.
• Under chronic inflammatory conditions, SAA1 can undergo proteolytic processing and misfolding, leading to the formation of amyloid fibrils and contributing to secondary (AA) amyloidosis.
• Human SAA1 is a member of the serum amyloid A family, which includes acute phase isoforms SAA1 and SAA2, sharing high sequence homology.
• Keywords from UniProt indicate involvement in amyloidosis, acute phase response, and reference proteome categories.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was generated against a synthetic peptide within the N-terminal region (amino acids 1-100) of human SAA1. This region is conserved across human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blot (WB): A band at approximately 12 kDa is expected for endogenous SAA1. Consider using positive controls such as liver lysates or plasma samples from acute phase-induced models.
• ELISA applications: The antibody can be used as a capture or detection reagent in sandwich ELISA for quantifying SAA1 in biological fluids. Pairing with another clone may be necessary; optimization is recommended.
• Due to its polyclonal nature, the antibody may detect multiple epitopes or low-level background; titration and appropriate blocking are advised.
• Although validated for human, mouse, and rat, reactivity in other species should be tested individually.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This Anti-SAA1 polyclonal antibody is part of CamelBio’s extensive portfolio of acute phase and inflammation-related antibodies, available in bulk with guaranteed lot-to-lot consistency. Beyond this product, CamelBio offers tailored sourcing solutions including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents to accelerate your assay development.