Anti-S6 Ribosomal Protein (RPS6) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62753

Core Product Specifications and Parameters

Biological Background: S6 Ribosomal Protein Function and Localization

• RPS6 is a core component of the 40S small ribosomal subunit.
  Related references: PMID:23636399, PMID:8706699
• It plays a critical role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
  Related references: PMID:17220279
• During ribosome biogenesis, RPS6 is part of the small subunit (SSU) processome and participates in pre-rRNA processing within the nucleolus.
  Related references: PMID:34516797
• Subcellular localization studies show RPS6 in the cytoplasm and nucleus, with prominent nucleolar enrichment.
• RPS6 is subject to extensive post-translational modifications, including phosphorylation, acetylation, ADP-ribosylation, hydroxylation, and ubiquitin-like conjugation, which may regulate its function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–130) of human RPS6; this sequence is highly conserved in mouse and rat, consistent with the reported cross-reactivity.
• For western blotting, a band at approximately 29 kDa is expected; however, post-translational modifications may cause slight shifts.
• In immunohistochemistry and immunofluorescence, validate fixation and permeabilization conditions to ensure detection of both cytoplasmic and nuclear/nucleolar populations.
• For ELISA applications, consider pairing with an alternative clone or a detection antibody recognizing a distinct epitope to avoid competition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. This anti-RPS6 antibody supports research into ribosome biology, cell growth control, and translation regulation. Beyond this product, CamelBio supplies validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to streamline your assay development.