Anti-S1PR5 Rabbit Polyclonal Antibody for WB, ELISA - Q9H228

Core Product Specifications and Parameters

Biological Background: S1PR5 Function and Localization

• S1PR5, also known as endothelial differentiation G protein-coupled receptor 8 (EDG8) or S1P receptor Edg-8, is a member of the G protein-coupled receptor family.
• It serves as a high-affinity receptor for the bioactive lysophospholipid sphingosine 1-phosphate (S1P), coupling to both Gi/o and G12 subclasses of heterotrimeric G proteins.
• S1PR5 is implicated in the regulation of radial glial cell transformation into astrocytes and may influence cellular proliferation in the central nervous system.
• The receptor localizes to the cell membrane, consistent with its role as a transmembrane signaling transducer.
• Expression is widespread but highest in the brain, particularly in white matter regions like the corpus callosum; it is also detected in spleen, peripheral blood leukocytes, placenta, lung, and aorta.
• Alternative splicing yields two isoforms: isoform 1 predominates in peripheral tissues, while isoform 2 is enriched in the brain, spleen, and peripheral blood leukocytes.
• S1PR5 is overexpressed in leukemic large granular lymphocytes, suggesting a potential role in hematologic malignancies.
• Post-translational modifications include glycosylation, palmitoylation, and phosphorylation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• For Western blot, the expected band size is approximately 42 kDa; consider using a positive control lysate from S1PR5-expressing cells, such as brain or leukocyte samples, and optimize antibody dilution.
• For ELISA, the antibody may be used as a capture or detection reagent; pairing with a matched detection antibody is recommended for sandwich ELISA development.
• Given the polyclonal nature, lot-to-lot consistency should be confirmed for critical quantitative assays.
• Cross-reactivity with other S1P receptor family members is not tested; validation in the specific experimental context is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, bridging the gap from concept to clinic. For S1PR5-related studies in neuroscience and immunology, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Whether you require rare target reagents or large-scale production support, CamelBio simplifies your procurement process.