Anti-S1PR3 Rabbit Monoclonal Antibody for WB, ELISA - Q99500

Core Product Specifications and Parameters

Biological Background: S1PR3 Function and Localization

• Sphingosine 1-phosphate receptor 3 (S1PR3), also designated as EDG3, LPB3, S1P3, or EDG-3, is a member of the G protein-coupled receptor family.
• It serves as a high-affinity receptor for the lysosphingolipid sphingosine 1-phosphate (S1P), a bioactive lipid mediator involved in numerous cellular processes.
• In rat hepatoma cells, S1PR3 has been shown to mediate S1P-induced cell proliferation and suppression of apoptosis, highlighting its role in cell survival signaling.
• S1PR3 localizes primarily to the cell membrane, consistent with its function as a transmembrane receptor.
• It is widely expressed across human tissues, with particularly high expression levels observed in the heart, placenta, kidney, and liver.
• The receptor undergoes post-translational modifications, including glycosylation and phosphorylation, and features multiple transmembrane helices characteristic of GPCRs.

Experimental Guidance and Technical Tips

• Because the immunogen corresponds to an N-terminal sequence, the antibody may preferentially recognize the native, unmodified form; performance on denatured samples (WB) should be verified.
• For Western blot, the predicted molecular weight is approximately 42 kDa, but glycosylation or other modifications may cause a shift; consider this during band identification.
• For ELISA, optimization of coating antigen and antibody concentration is recommended, especially when using recombinant protein or cell lysates.
• Cross-reactivity with mouse and rat S1PR3 has been observed; however, sequence homology should be checked for other species if needed.
• Always validate in the relevant sample system, as post-translational modifications or expression levels may vary across tissues and cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop sourcing partner for IVD raw materials, technical services, and consulting. Our anti-S1PR3 monoclonal antibody supports research into sphingolipid signaling and related diseases. We also offer validated antibody pairs, bulk ancillary reagents, and sourcing for rare targets to accelerate your diagnostic development from concept to clinic.