Anti-S100/S100A1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P23297

Core Product Specifications and Parameters

Biological Background: Protein S100-A1 Function and Localization

• Protein S100-A1 (S100 calcium-binding protein A1, also known as S-100 protein alpha chain or S100A) is a member of the S100 family of EF-hand calcium-binding proteins.
• Functions as a small calcium-binding protein that plays critical roles in Ca²⁺ homeostasis, chondrocyte biology, and cardiomyocyte regulation by binding calcium and triggering conformational changes that enable interactions with specific target proteins. Related references: PMID:12804600 PMID:23351007 PMID:22399290
• In cardiomyocytes, S100-A1 regulates sarcoplasmic reticulum Ca²⁺ cycling and mitochondrial function through interaction with ryanodine receptors (RYR1, RYR2), sarcoplasmic reticulum Ca²⁺-ATPase (ATP2A2), and mitochondrial F1-ATPase. Related references: PMID:12804600
• S100-A1 facilitates diastolic Ca²⁺ dissociation and improves myofilament mechanics, thereby enhancing relaxation during diastole. Related references: PMID:11717446
• Subcellularly, S100-A1 localizes to the cytoplasm, sarcoplasmic reticulum, and mitochondrion.
• Tissue specificity: Highly prevalent in heart, with lower expression in skeletal muscle and brain. Related references: PMID:12804600 PMID:1384693
• The protein undergoes post-translational modifications including glutathionylation and S-nitrosylation, and contains EF-hand repeats characteristic of calcium-binding proteins.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-94 of human S100A1 (NP_006262.1); thus, the polyclonal antibody is expected to recognize multiple epitopes across the full-length or near-full-length protein.
• For Western blot (WB), S100A1 has a predicted molecular weight of ~11 kDa (UniProt: 10.5 kDa); ensure appropriate low-molecular-weight protein transfer and blocking conditions.
• In immunohistochemistry (IHC-P), strong expression is anticipated in heart tissue; consider using heart sections as a positive control and validate with relevant sample systems.
• For ELISA applications, the antibody’s polyclonal nature may provide robust signal amplification; however, assay conditions should be optimized empirically.
• As cross-reactivity is noted with human, mouse, and rat, species-specific sequence alignment should be verified to ensure expected reactivity in experimental models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of research-grade antibodies, including this S100/S100A1 rabbit polyclonal antibody, to support cardiac biology and calcium signaling research. Our supply capabilities encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, enabling seamless transition from development to production. Leverage CamelBio's integrated supply chain to streamline your diagnostic assay development with reliable and well-characterized reagents.