Anti-S100/S100A1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P23297

Core Product Specifications and Parameters

Biological Background: S100A1 Function and Localization

• Protein S100-A1, also known as S-100 protein alpha chain, is a small calcium-binding protein of the S100 family, encoded by the S100A1 gene.
• Plays a crucial role in calcium homeostasis by binding Ca2+ and undergoing conformational changes to interact with target proteins. Related references: PMID:23351007 PMID:22399290
• In cardiomyocytes, it regulates sarcoplasmic reticulum Ca2+ cycling and mitochondrial function via interactions with ryanodine receptors (RYR1/RYR2), SERCA2a (ATP2A2), and mitochondrial F1-ATPase. Related references: PMID:12804600
• Facilitates diastolic Ca2+ dissociation and improves myofilament mechanics to enhance relaxation during diastole. Related references: PMID:11717446
• Subcellularly, it localizes to the cytoplasm, sarcoplasmic reticulum, and mitochondrion.
• Highly expressed in heart, with lower levels in skeletal muscle and brain.
• Post-translational modifications include glutathionylation and S-nitrosylation; belongs to the calcium-binding protein family with EF-hand repeats.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 1–94 of human S100A1, suggesting the antibody likely recognizes the N-terminal region.
• For Western blotting, consider a dilution range typical for rabbit monoclonal antibodies and validate in tissue lysates with high S100A1 expression (e.g., heart, skeletal muscle, brain).
• In immunohistochemistry (IHC-P), optimize fixation and antigen retrieval methods for the target tissue; use appropriate controls.
• For immunofluorescence (IF/ICC), ensure permeabilization steps are compatible with the subcellular localization (cytoplasmic, SR, mitochondrial).
• ELISA performance may vary; validate with recombinant S100A1 protein or relevant biological samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a streamlined, one-stop sourcing solution for IVD raw materials, covering every stage from concept to clinic. For S100A1-focused research and assay development, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw‑material sourcing. Our flexible supply capabilities help you accelerate cardiovascular biomarker and companion diagnostics programs with confidence.