Anti-S100P Rabbit Monoclonal Antibody for WB, IF/ICC, and ELISA - P25815

Core Product Specifications and Parameters

Biological Background: S100P Function and Localization

• S100P is a member of the S100 family of calcium-binding proteins with EF-hand motifs, also known as MIG9 (Migration-inducing gene 9 protein) and S100E.
• Functions as a calcium sensor, modulating cellular calcium signaling by interacting with downstream effectors such as EZR (ezrin) and PPP5C (serine/threonine-protein phosphatase 5).
• Plays a role in epithelial cell microvilli formation and may regulate cytoskeletal dynamics at the cell cortex.
• Stimulates cell proliferation in an autocrine manner via activation of the receptor for advanced glycation end products (RAGE), potentially contributing to tumor growth.
• Localizes to the nucleus, cytoplasm, and cell projections, including microvillus membranes, reflecting its dynamic signaling roles.
• Tissue distribution: widely expressed, with higher levels in placenta, heart, lung, skeletal muscle, spleen, and leukocytes; notably upregulated in pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias.
• Alternative names (MIG9, S100E) hint at involvement in cell migration and epithelial biology.
• The theoretical molecular weight is approximately 10.4 kDa, consistent with the ~10 kDa band observed in Western blot.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-95, encompassing the N-terminal EF-hand domains. Antibody binding may be sensitive to calcium-induced conformational changes; consider performing experiments in the presence and absence of calcium to assess epitope accessibility.
• For Western blot, expect a band near 10 kDa. Use a low molecular weight marker range and consider a 15% or gradient SDS-PAGE gel for optimal resolution of small proteins.
• For IF/ICC, S100P localizes to both nucleus and cytoplasm, with enrichment at membrane projections. Validate in relevant cell line models such as pancreatic cancer cells (e.g., PANC-1, BxPC-3) where expression is elevated.
• For ELISA, use recombinant human S100P as a positive control. Ensure coating buffer and wash conditions are compatible with calcium-binding protein stability.
• Include appropriate negative controls (e.g., tissue lysates from brain or testis, where S100P is low or absent) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated provider of IVD raw materials, CamelBio offers bulk monoclonal antibodies, including this S100P rabbit mAb, to support research and diagnostic development in calcium signaling and oncology. Our portfolio encompasses validated antibody pairs, recombinant proteins, and ancillary reagents, streamlining your supply chain from discovery to clinical application. For projects requiring rare target reagents or custom sourcing, our team bridges the gap between concept and clinic with reliable, one-stop access.