Anti-S100P Polyclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - P25815

Core Product Specifications and Parameters

Biological Background: S100P Function and Localization

• Protein S100-P, also known as Migration-inducing gene 9 protein (MIG9) or S100-E, belongs to the S100 family of calcium-binding proteins characterized by EF-hand motifs.
• It functions as a calcium sensor, participating in intracellular calcium signaling by interacting with target proteins like EZR and PPP5C in a calcium-dependent manner.
• S100P plays a role in microvilli formation in epithelial cells and can stimulate cell proliferation via autocrine activation of the receptor for advanced glycation end products (RAGE).
• Subcellularly, S100P localizes to the nucleus, cytoplasm, and cell projections, particularly associated with the microvillus membrane.
• Tissue expression is widespread except in brain, testis, and small intestine, with higher levels in placenta, heart, lung, skeletal muscle, spleen, and leukocytes; it is notably upregulated in pancreatic ductal adenocarcinomas and intraepithelial neoplasias.
• As a metal-binding protein with calcium-dependent conformational changes, S100P is involved in signaling pathways that regulate cytoskeletal organization and cellular proliferation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–95 of human S100P, targeting the N-terminal region; consider this when designing epitope-based assays.
• As a polyclonal antibody, it offers robust signal in WB, IP, and IHC-P; validate in the appropriate human tissue or cell line systems.
• For ELISA applications, the antibody may be used as either capture or detection reagent; empirical optimization of coating and detection conditions is recommended.
• For immunofluorescence-paraffin (IF-P) and IHC-P, include appropriate antigen retrieval steps and verify staining patterns against known controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing platform for IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality reagents such as this anti-S100P polyclonal antibody. In the field of oncology and calcium signaling research, we facilitate procurement of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target raw-material sourcing. Let CamelBio bridge your development needs from concept to clinic with reliable supply and technical guidance.