Anti-S100B Rabbit Polyclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - P04271

Core Product Specifications and Parameters

Biological Background: S100B Function and Localization

• S100B (S-100 protein beta chain) is a small zinc- and calcium-binding protein highly expressed in astrocytes, where it constitutes one of the most abundant soluble brain proteins.
  Related references: PMID:20950652 PMID:6487634
• The protein weakly binds calcium but tightly binds zinc, with distinct binding sites for each ion on each monomer; physiological potassium concentrations antagonize both divalent cation binding.
  Related references: PMID:20950652 PMID:6487634
• S100B acts as a neurotrophic factor, promoting astrocytosis and axonal proliferation, and is also involved in sympathetic innervation of thermogenic adipose tissue as an adipocyte-derived neurotrophic factor.
• It may assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization.
  Related references: PMID:20351179
• S100B can mediate calcium-dependent regulation by interacting with TPR-containing proteins and modulating their activity.
  Related references: PMID:22399290
• Post-myocardial infarction, interaction with AGER may trigger myocyte apoptosis via ERK1/2 and p53/TP53 signaling.
• Subcellular localization includes cytoplasm, nucleus, and secretion.
• Although predominantly a brain protein, S100B is also detected in a variety of other tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human S100B (amino acids 1-92), which is highly conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For Western blotting, use a low molecular weight marker and optimize transfer conditions given the ~11 kDa protein size.
• For immunohistochemistry (IHC-P), consider citrate-based antigen retrieval and validate in the relevant tissue type; for immunofluorescence (IF-P), include appropriate permeabilization steps.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized partner for diagnostic manufacturers and research institutes, offering a comprehensive range of IVD raw materials and technical services. This S100B rabbit polyclonal antibody supports research and assay development in neurobiology and neurotrophic factor signaling. Beyond individual antibodies, we supply validated antibody pairs, monoclonal and polyclonal formats, bulk ancillary reagents, and provide rare-target raw-material sourcing to streamline your transition from concept to clinic.