Anti-S100A9 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P31725

Core Product Specifications and Parameters

Biological Background: S100A9 Function and Localization

• S100A9 (calgranulin-B, MRP-14, p14) is a member of the S100 family of calcium-binding proteins.

• It functions as a calcium- and zinc-binding protein that prominently regulates inflammatory processes and immune responses.

  Related references: PMID:15331440 PMID:17767165 PMID:18403730

• Intracellularly, S100A9 facilitates leukocyte arachidonic acid trafficking, modulates the tubulin-dependent cytoskeleton during phagocyte migration, and activates the neutrophilic NADPH-oxidase.

  Related references: PMID:15331440

• As part of calprotectin (S100A8/A9 heterodimer), it exerts pro-inflammatory, antimicrobial, oxidant-scavenging, and apoptosis-inducing extracellular activities.

  Related references: PMID:21382888

• It acts as a danger-associated molecular pattern (DAMP), stimulating innate immune cells via TLR4 and AGER, leading to MAP-kinase and NF-kappa-B activation and amplification of inflammation.

  Related references: PMID:17767165 PMID:18403730 PMID:19402754

• S100A9 has antimicrobial activity towards bacteria and fungi, likely through Zn2+ chelation.

• Subcellularly, S100A9 is found secreted, as well as in the cytoplasm, associated with the cytoskeleton and cell membrane.

• Post-translational modifications include acetylation and phosphorylation; it belongs to a family of metal-binding proteins with roles in chemotaxis, apoptosis, and autophagy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-113 of mouse S100A9; this N-terminal region may influence epitope recognition in different applications.

• For Western blot, expect a band near 13 kDa under reducing conditions; validate using positive controls such as mouse spleen or bone marrow lysates.

• For IHC-P, consider antigen retrieval and test with tissues known to express high levels of S100A9, e.g., inflamed or myeloid-rich samples.

• For ELISA, the polyclonal nature allows use as either capture or detection reagent; pairing with a monoclonal antibody may improve specificity.

• The antibody cross-reacts with human S100A9; confirm reactivity in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable IVD raw materials, including validated antibodies, antigens, and ancillary reagents. This anti-S100A9 polyclonal antibody supports research into inflammatory disease markers and immune response pathways. Contact us for bulk orders, technical consultation, and customized sourcing solutions for your assay development needs.