Anti-S100A8 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P27005

Core Product Specifications and Parameters

Biological Background: S100A8 Function and Localization

• S100A8 is a small calcium- and zinc-binding protein (10295 Da) belonging to the S100 family, also known as calgranulin-A, MRP-8, p8, and CP-10.
• It plays a prominent role in the regulation of inflammatory processes and innate immune responses, primarily by inducing neutrophil chemotaxis and adhesion.
• Intracellularly, as part of the S100A8/A9 (calprotectin) complex, it facilitates leukocyte arachidonic acid trafficking, modulates tubulin-dependent cytoskeleton reorganization during phagocyte migration, and activates the NADPH-oxidase by promoting enzyme complex assembly and transferring arachidonic acid to the complex.
• Extracellularly, S100A8 acts as an alarmin or DAMP molecule, binding to pattern recognition receptors such as TLR4 and AGER, which triggers MAP kinase and NF-κB signaling to amplify pro-inflammatory cascades; it also possesses direct antimicrobial activity via Zn²⁺ chelation.
• The protein can induce cell death through autophagy and apoptosis via cross-talk between mitochondria and lysosomes involving ROS and BNIP3, and it regulates neutrophil survival through ITGAM/ITGB and TLR4-mediated MEK-ERK pathways.
• According to UniProt keywords and functional data, S100A8 is involved in S-nitrosylation events as part of the iNOS-S100A8/A9 transnitrosylase complex, contributing to site-selective modification of target proteins.
• Subcellularly, S100A8 is found in the cytoplasm, associated with the cytoskeleton, at the cell membrane, and is also secreted into the extracellular space.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant protein covering amino acids 1-89 of mouse S100A8 (NP_038678.1). Given this sequence corresponds to nearly the full-length protein (predicted molecular weight ~10 kDa), the antibody should recognize the native form. For Western blot, expect a band around 10–12 kDa; reduction and denaturation may help expose the epitope fully.
• As a polyclonal antibody, it may have slight lot-to-lot variation; consider validating performance with a known positive control such as mouse bone marrow or spleen lysate, or cells stimulated to express S100A8 (e.g., LPS-treated macrophages).
• For immunohistochemistry (IHC-P), antigen retrieval conditions (e.g., citrate buffer pH 6.0 heat-induced epitope retrieval) should be optimized, and endogenous peroxidase quenching may be necessary. Check cross-reactivity with rat tissues as noted.
• In ELISA applications, coat recombinant S100A8 or use a sandwich format with a matched antibody pair; confirm specificity with relevant negative controls.

CamelBio: Your One-Stop Sourcing Bridge

This S100A8 antibody supports inflammation and innate immunity research, and CamelBio streamlines the path from target discovery to diagnostic development. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal panels, bulk ancillary reagents, and flexible sourcing for rare targets. From concept to clinic, CamelBio is your end-to-end partner for IVD raw materials—reducing development timelines without compromising on quality or reliability.