Anti-S100A8 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P05109

Core Product Specifications and Parameters

Biological Background: S100A8 Function and Localization

• Protein S100-A8, also known as calgranulin-A or MRP8, is a small calcium- and zinc-binding protein of the S100 family.
• It forms a heterocomplex with S100A9 known as calprotectin, which exerts pleiotropic intracellular and extracellular functions in inflammation and immune response.
• Intracellularly, it facilitates arachidonic acid trafficking, modulates cytoskeleton during phagocyte migration, and activates NADPH-oxidase.
• Participates in regulatory T-cell differentiation together with CD69. Related references: PMID:26296369
• Extracellular S100A8 acts as a DAMP/alarmin, binding TLR4 and AGER to amplify pro-inflammatory cascades.
• Possesses antimicrobial activity against bacteria and fungi likely via Zn2+ chelation.
• Predominantly expressed in myeloid cells (neutrophils and monocytes), strongly upregulated in inflammatory conditions and many tumors.
• Subcellularly found in cytoplasm, cytoskeleton, cell membrane, and secreted.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-93 of human S100A8, covering the full-length protein. As a rabbit polyclonal antibody, it recognizes multiple epitopes and is validated for use with human, mouse, and rat samples.
• For Western blotting, consider a 12–15% SDS-PAGE gel to resolve the ~11 kDa band and use appropriate low molecular weight markers.
• For immunohistochemistry and immunofluorescence, antigen retrieval may be required; optimize fixation and blocking conditions for your specific tissue type.
• This antibody can be used in ELISA for quantitative detection of S100A8 in serum or culture supernatants.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, connecting diagnostic developers with validated antibodies and reagents. Our portfolio includes optimized polyclonal antibodies like this anti-S100A8 antibody, suitable for multi-species inflammation and cancer biomarker research. We supply bulk ancillary reagents and can source rare-target raw materials to support your IVD development from concept to clinic.