Anti-S100A8 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P05109

Core Product Specifications and Parameters

Biological Background: S100A8 Function and Localization

• S100A8, also known as Calgranulin-A, MRP8, or CFAG, is a calcium- and zinc-binding protein of the S100 family, predominantly expressed in myeloid cells such as neutrophils and monocytes.
• Intracellularly, as part of the calprotectin heterodimer (S100A8/A9), it facilitates arachidonic acid trafficking, modulates cytoskeleton dynamics during phagocyte migration, and activates the neutrophilic NADPH-oxidase by aiding enzyme complex assembly and transferring arachidonic acid.
• Extracellularly, S100A8 acts as a pro-inflammatory alarmin/DAMP, recruiting leukocytes and promoting cytokine/chemokine production by binding to TLR4 and AGER, thereby amplifying inflammatory cascades via MAPK and NF-kB pathways.
• It exhibits antimicrobial activity against bacteria and fungi likely through Zn²⁺ chelation, and can induce autophagy and apoptosis via ROS and BNIP3.
• S100A8 participates in regulatory T-cell differentiation together with CD69. Related references: PMID:26296369
• Subcellularly, S100A8 is found in the cytoplasm, associated with the cytoskeleton, at the cell membrane, and is also secreted.
• It is strongly upregulated in many cancers and inflammatory diseases, and has been found in high concentrations at local inflammation sites and in serum of patients with conditions like rheumatoid arthritis, cystic fibrosis, and various cancers.
• Keywords include: antimicrobial, apoptosis, autophagy, calcium, chemotaxis, immunity, inflammatory response, innate immunity, membrane, metal-binding, S-nitrosylation, secreted, zinc.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning the N-terminal region (aa 1-93), which may target a conserved epitope, supporting cross-reactivity with mouse and rat S100A8 as indicated.
• For WB, the predicted molecular weight is ~11 kDa; consider using a low molecular weight marker and appropriate gel percentage for small proteins.
• For IHC-P, validate in inflamed or tumor tissues where S100A8 expression is upregulated; antigen retrieval conditions may need optimization.
• The antibody is suitable for ELISA; consider pairing with another S100A8 antibody for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing solutions for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-S100A8 antibody supports research into inflammatory diseases, cancer biomarkers, and immune response mechanisms. We assist with one-stop procurement from concept to clinic, ensuring reliable supply for assay development.