Anti-S100A7/Psoriasin Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P31151

Core Product Specifications and Parameters

Biological Background: S100A7/Psoriasin Function and Localization

• S100A7, also known as psoriasin or S100 calcium-binding protein A7, is encoded by the S100A7 gene (aliases PSOR1, S100A7C).
• It is a member of the S100 family of calcium-binding proteins, characterized by EF-hand motifs that mediate calcium and zinc binding.
• S100A7 localizes to the cytoplasm and is also secreted extracellularly, suggesting both intracellular and extracellular functions.
• Tissue expression includes fetal ear, skin, and tongue, with strong upregulation in psoriatic epidermis, implicating it in inflammatory skin conditions.
• In bladder squamous cell carcinoma, S100A7 is highly expressed in urine, indicating potential as a non-invasive biomarker.
• Post-translational modifications include acetylation and formation of disulfide bonds, which may regulate its structure and function.
• Keywords associated with S100A7: 3D-structure, calcium, zinc, metal-binding, secreted, and direct protein sequencing.

Experimental Guidance and Technical Tips

• The recombinant protein immunogen suggests potential recognition of native structure; validate performance under your specific assay conditions.
• For western blotting, the target migrates at approximately 11 kDa; include a molecular weight marker and validate with known expressing cells or tissues.
• In IHC-P, test different antigen retrieval methods and antibody dilutions using psoriatic skin or squamous cell carcinoma samples as positive controls.
• For ELISA, optimize coating antigen and antibody concentrations; use recombinant S100A7 as standard.
• The antibody is reported to cross-react with human; verify cross-reactivity in other species experimentally if required.
• As a rabbit monoclonal antibody, expect high specificity and reproducibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with reliable raw materials for inflammation and oncology biomarker development. This S100A7/Psoriasin antibody is part of our comprehensive catalog of monoclonal antibodies, validated pairs, and ancillary reagents. Contact us to source bulk quantities, custom formulations, or rare-target raw materials for your IVD projects.