Anti-S100A6 Rabbit Polyclonal Antibody (KO Validated) for WB, IF/ICC, ELISA - P06703

Core Product Specifications and Parameters

Biological Background: S100A6 Function and Localization

• Protein S100-A6 (also known as Calcyclin, PRA, 2A9, 5B10, CABP, CACY, S10A6) is a small calcium-binding protein of the S100 family, encoded by the S100A6 gene (GeneID: 6277).
• Acts as a calcium sensor and modulator, binding two calcium ions cooperatively, and is involved in cellular calcium signaling.
• Through interaction with proteins containing tetratricopeptide repeat (TPR) domains, it indirectly regulates reorganization of the actin cytoskeleton and cell motility.
• Localizes to the nucleus envelope, cytoplasm, and cell membrane, indicating multiple sites of action.
• Subject to post-translational modifications including acetylation and phosphorylation, which may modulate its function.
• During microbial infection, facilitates attachment and invasion of Toxoplasma gondii parasites.
• Belongs to the S100 family of EF-hand calcium-binding proteins.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human S100A6 protein (aa 1-90), so the polyclonal antibody is expected to recognize multiple epitopes, potentially enhancing sensitivity in western blotting and ELISA.
• For western blotting, a band at ~10 kDa is expected. Ensure proper transfer conditions and use a low molecular weight marker to confirm specificity.
• In IF/ICC, consider the reported localization to the nucleus envelope, cytoplasm, and cell membrane. Optimize fixation and permeabilization conditions for your sample.
• Cross-reactivity with mouse and rat is reported; validate in the relevant species, as sequence homology may influence binding affinity.
• For ELISA, test as capture or detection antibody after confirming reactivity with native antigen in your specific buffer system.
• As a KO-validated antibody, it is useful for verifying S100A6 knockout efficiency in human cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to high-quality IVD raw materials and technical support. For S100A6, a calcium-binding protein involved in cell motility and host-pathogen interactions, we supply validated polyclonal antibodies and can source complementary reagents. Whether you need bulk antibody production, rare target sourcing, or custom development, CamelBio bridges your concept to clinical application.