Anti-S100A6 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P06703

Core Product Specifications and Parameters

Biological Background: S100A6 Function and Localization

• S100A6 (Calcyclin) is a member of the S100 family of small, acidic calcium-binding proteins that contains two EF-hand motifs and functions in calcium-dependent signal transduction.
• It acts as a calcium sensor and modulator, contributing to cellular calcium signaling by interacting with downstream target proteins such as TPR-containing proteins, thereby influencing actin cytoskeleton reorganization and cell motility.
• The protein binds two calcium ions cooperatively, and its activity is mediated by conformational changes upon calcium binding.
• Subcellular localization includes the nuclear envelope, cytoplasm, and cell membrane, reflecting its involvement in diverse signaling compartments.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its function and interactions.
• (Microbial infection) S100A6 facilitates the attachment and invasion of Toxoplasma gondii parasites, suggesting a role in host-pathogen interactions.
• Keywords from UniProt include: 3D-structure, Acetylation, Calcium, Cell membrane, Cytoplasm, Direct protein sequencing, Membrane, Metal-binding, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Repeat.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-90 of human S100A6, nearly the full-length protein; thus, the antibody is expected to recognize the native and denatured forms in multiple applications.
• For Western blotting, expect a band at ~10 kDa; consider using low molecular weight markers and a membrane with small pore size (e.g., 0.2 µm) to prevent transfer loss.
• Validate antibody specificity by comparing signal in S100A6-positive and negative cell lines or tissues; S100A6 expression is widespread but elevated in certain cancers.
• For immunohistochemistry and immunofluorescence, optimize antigen retrieval and permeabilization steps based on target localization (nuclear envelope, cytoplasm, membrane); calcium chelators (EDTA/EGTA) in buffers may affect antigen conformation.
• For immunoprecipitation, note that calcium ions may be required for native protein conformation; consider adding calcium to lysis and wash buffers if detection is conformation-sensitive.
• Cross-reactivity with mouse and rat S100A6 is confirmed; however, always verify in your specific experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibody pairs, bulk polyclonal antibodies, and ancillary reagents. This anti-S100A6 polyclonal antibody supports research into calcium signaling, cancer biology, and host-pathogen interactions. As your sourcing partner, CamelBio offers one-stop solutions from concept to clinic, ensuring reliable supply for your assay development and kit production needs, including rare and specialized targets.