Anti-S100A6 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P06703

Core Product Specifications and Parameters

Biological Background: S100A6 Function and Localization

• S100A6, also known as calcyclin, is a member of the S100 family of small EF-hand calcium-binding proteins, characterized by two calcium-binding sites.
• It functions as a cytoplasmic and nuclear calcium sensor, binding two calcium ions cooperatively in response to elevated intracellular calcium levels, and transduces calcium signals by interacting with target proteins.
• Through its interaction with proteins such as TPR-containing proteins, S100A6 contributes to the reorganization of the actin cytoskeleton and regulation of cell motility, impacting processes like cell migration and proliferation.
• Subcellularly, S100A6 exhibits a dynamic localization pattern, being found at the nuclear envelope, in the cytoplasm, and associated with the cell membrane, which supports its role in broad signal transduction pathways.
• Post-translational modifications, including acetylation and phosphorylation, have been identified and likely modulate its activity, stability, or protein-protein interactions.
• Beyond normal cellular roles, S100A6 is hijacked during microbial infection; it facilitates the attachment and invasion of Toxoplasma gondii parasites, linking it to host-pathogen interactions.

Experimental Guidance and Technical Tips

• For Western blotting, a specific band at approximately 10 kDa is expected. Consider using low molecular weight markers and optimized transfer conditions (e.g., wet transfer with 0.2 µm membrane) to avoid small protein loss.
• In immunohistochemistry (IHC-P), antigen retrieval protocols (e.g., heat-induced epitope retrieval) may enhance staining. Validate with appropriate tissue controls and compare with known expression patterns.
• For immunofluorescence (IF/ICC), since S100A6 localizes to both cytoplasm and nucleus, permeabilization is critical. Adjust fixation and permeabilization steps to ensure access to nuclear compartments.
• The antibody’s cross-reactivity with mouse and rat makes it suitable for rodent model studies; however, always confirm specificity in the relevant species by comparing results with knockout controls or siRNA-mediated knockdown where possible.
• ELISA applications may require optimization of coating conditions and detection ranges; consider pairing with a matched detection antibody for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, clinical labs, and research groups to streamline IVD raw material supply, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents that accelerate assay development. For calcium signaling, cancer biology, or infectious disease projects focused on S100A6, we provide specialized sourcing support to connect your workflow with high-quality critical raw materials—from initial research to clinical application. Let our procurement expertise handle rare target sourcing and reliable supply chain logistics so you can focus on innovation.