Anti-S100A3 Rabbit Polyclonal Antibody for WB and ELISA - P33764

Core Product Specifications and Parameters

Biological Background: S100A3 Function and Localization

• Protein S100-A3 (also known as Protein S-100E, S100 calcium-binding protein A3) is a member of the S100 family of calcium-binding proteins, encoded by the S100A3 (S100E) gene in humans.
• S100A3 binds both calcium and zinc ions, suggesting a role in metal ion-dependent signaling or structural processes in specific cell types.
• The protein is involved in calcium-dependent cuticle cell differentiation, contributing to hair shaft formation and the establishment of the hair cuticular barrier.
• Subcellularly, S100A3 is localized to the cytoplasm, where it may interact with other proteins or metal ions to exert its function.
• Tissue specificity studies indicate that S100A3 expression is skin-specific, with high levels observed at the inner endocuticle of hair fibers.
• Post-translational modifications of S100A3 include acetylation and citrullination, and it contains disulfide bonds, which could impact its structure and function. Keywords also include "Repeat" and "Reference proteome".

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant fusion protein encompassing amino acids 1-101 of human S100A3. This region covers nearly the entire protein (101 out of 101 amino acids), suggesting the antibody may recognize multiple epitopes.
• For Western blot (WB), consider loading 10-20 µg of total protein from skin or hair follicle lysates, as S100A3 is skin-specific. Validate the antibody in the relevant sample system (human, mouse, or rat) as per your experimental needs.
• For ELISA, coating with the recombinant immunogen or S100A3-containing tissue extracts may be used. Include appropriate positive and negative controls to ensure specificity.
• Since cross-reactivity is reported for human, mouse, and rat, the antibody could be used in comparative studies across these species, but species-specific validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibodies for skin biology and hair research. In addition to this anti-S100A3 polyclonal antibody, we offer optimized monoclonal antibodies, antibody pairs, and bulk ancillary reagents to support your assay development. Our one-stop sourcing services also cover rare target raw-material procurement and custom antibody generation, streamlining your path from concept to clinic.