Anti-S100A16 Rabbit Polyclonal Antibody for WB, ELISA - Q96FQ6

Core Product Specifications and Parameters

Biological Background: S100A16 Function and Localization

• S100A16 (Protein S100-A16) is a member of the S100 calcium-binding protein family, encoded by the S100A16 gene (also known as S100F).
• S100A16 binds one calcium ion per monomer with high affinity, functioning as a calcium sensor. Related references: PMID:17030513
• It is involved in adipocyte biology: it can promote adipocyte differentiation in vitro, and its overexpression in preadipocytes enhances proliferation and adipogenesis while reducing insulin-stimulated glucose uptake.
• The protein localizes to the nucleus, nucleolus, and cytoplasm, suggesting roles in both nuclear and cytoplasmic processes.
• S100A16 exhibits ubiquitous tissue expression, with highest levels in esophagus, adipose tissue, and colon; lower expression in lung, brain, pancreas, and skeletal muscle. Expression is upregulated in several tumor types (bladder, lung, thyroid, pancreas, ovary) and is found in astrocytes. Related references: PMID:14684152 PMID:17030513
• The protein contains calcium-binding EF-hand repeats and also binds zinc, as indicated by proteomics and structural studies.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of S100A16 is approximately 12 kDa; use a high-percentage SDS-PAGE gel (e.g., 15%) and low-molecular-weight markers for optimal resolution.
• Given the nuclear and cytoplasmic localization of S100A16, consider subcellular fractionation to enrich target protein in complex samples.
• Test the antibody in relevant human, mouse, or rat cell lines or tissues known to express S100A16, such as adipocytes, esophageal epithelium, or tumor lines.
• For ELISA, titrate the antibody as a capture or detection reagent and validate with recombinant S100A16 or native protein in lysates; pair with a matched detection antibody for sandwich ELISA if needed.
• Since S100A16 is a calcium-binding protein, include calcium-chelating agents (e.g., EDTA) in lysis buffers with caution, as they may affect protein conformation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop procurement platform for IVD raw materials, supporting research in adipogenesis, cancer biomarker discovery, and calcium signaling pathways. We supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to streamline assay development. Contact us for assistance in sourcing rare-target antibodies and custom generation services.