Anti-S100A12 Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P80511

Core Product Specifications and Parameters

Biological Background: S100A12 Function and Localization

• S100A12 is a calcium-, zinc- and copper-binding protein of the S100 family, also known as EN-RAGE, calgranulin-C (CAGC), MRP-6, and p6.
• It plays a prominent role in regulating inflammatory processes and immune response by acting as a DAMP/alarmin molecule.
• Upon binding to RAGE (AGER), S100A12 activates MAP-kinase and NF-kappa-B pathways, inducing pro-inflammatory cytokines and upregulating adhesion molecules ICAM1 and VCAM1.
• It chemoattracts monocytes and mast cells, stimulates mast cell degranulation, and promotes leukocyte recruitment to sites of inflammation.
• S100A12 inhibits matrix metalloproteinases (MMP2, MMP3, MMP9) by chelating zinc from their active sites.
• It has antimicrobial properties, including antifungal activity against C. albicans and activity against E. coli and P. aeruginosa.
• Predominantly expressed in neutrophils, monocytes, activated macrophages, and eosinophils in asthmatic airways; elevated serum levels are associated with inflammatory disorders.
• Subcellular localization includes secreted, cytoplasmic, cytoskeletal, and cell membrane compartments.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length S100A12 (aa 1-92). Ensure protein extraction conditions preserve the epitope.
• For Western blot (WB), note the low molecular weight (~10 kDa). Consider using high-percentage gels and optimize transfer conditions.
• In IHC-P, antigen retrieval may be required. Validate staining in positive control tissues such as inflamed tonsil or colon.
• For IF-P, expect localization primarily in the cytoplasm and at the membrane; confirm with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. This anti-S100A12 antibody supports inflammation and immunology research; our team can help source additional rare targets or bulk quantities to meet your project needs. From concept to clinic, CamelBio streamlines your raw-material procurement.