Anti-S100A12 Polyclonal Antibody for IHC-P, ELISA - P80511

Core Product Specifications and Parameters

Biological Background: S100A12 Function and Localization

• Protein S100-A12, also known as calgranulin-C, EN-RAGE (Extracellular Newly Identified RAGE-binding Protein), CAAF1, MRP-6, and p6, is a member of the S100 calcium-binding protein family.
• It plays a central role in inflammatory and immune responses by functioning as a danger-associated molecular pattern (DAMP) molecule, binding to the receptor for advanced glycation endproducts (AGER/RAGE) and activating MAP-kinase and NF-kappa-B pathways to promote cytokine production and up-regulate adhesion molecules like ICAM1 and VCAM1.
• Acts as a chemoattractant for monocytes and mast cells, and can stimulate mast cell degranulation, amplifying leukocyte recruitment to inflammation sites.
• Exhibits calcium-, zinc-, and copper-binding properties; it inhibits matrix metalloproteinases (MMP2, MMP3, MMP9) by chelating zinc from their active sites.
• Possesses antimicrobial activities: displays filariacidal and filariastatic effects, and its derivative calcitermin has antifungal activity against Candida albicans and antibacterial activity against E. coli and P. aeruginosa.
• Predominantly expressed in neutrophils, monocytes, and activated macrophages; elevated serum levels are observed in inflammatory disorders such as rheumatoid arthritis, Crohn's disease, and Kawasaki disease.
• Subcellularly, it is found secreted, in the cytoplasm, associated with the cytoskeleton, and at the cell membrane.
• Keywords: antibiotic, antimicrobial, calcium-binding, inflammatory response, innate immunity, metal-binding.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human S100A12 protein (amino acids 1-92). As a rabbit polyclonal antibody, it recognizes multiple epitopes, which may enhance detection sensitivity in denatured conditions.
• For IHC-P, consider using antigen retrieval methods (heat-induced) and validate staining in human tissues with known high S100A12 expression, such as inflamed synovium, colonic mucosa, or sites of neutrophil infiltration.
• For ELISA, the antibody can be used as a capture or detection reagent; pair with a compatible secondary antibody and use recombinant S100A12 as a standard for quantification.
• Cross-reactivity has been confirmed only with human S100A12; for other species, validate cross-reactivity experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to a comprehensive range of IVD raw materials. For inflammation and innate immunity research, we supply validated antibody pairs, optimized polyclonal antibodies like this anti-S100A12, and bulk ancillary reagents to support assay development. Our one-stop sourcing bridges the gap from concept to clinic with tailored technical services and consultative support.