Anti-S100A11 Polyclonal Antibody for WB, IF/ICC, ELISA - P31949

Core Product Specifications and Parameters

Biological Background: S100A11 Function and Localization

• Protein S100-A11 (calgizzarin/MLN70/S100C) is a small, acidic calcium-binding protein belonging to the S100 family, characterized by two EF-hand motifs.
• The primary biological role of S100A11 is to facilitate keratinocyte differentiation and cornification, a critical process in the formation of the epidermal barrier.
• Upon calcium binding, S100A11 undergoes conformational changes that enable interaction with various target proteins, influencing cellular processes such as cytoskeletal dynamics and signal transduction.
• The protein is distributed in both the cytoplasm and the nucleus, indicative of its involvement in cytoplasmic as well as nuclear regulatory functions.
• Post-translational modifications including acetylation, phosphorylation, and disulfide bond formation likely modulate its stability, localization, and activity.
• S100A11 is broadly conserved and is implicated in cell cycle progression, cytoskeletal organization, and calcium signaling pathways.

Experimental Guidance and Technical Tips

• The immunogen used spans the full-length human S100A11 protein (aa 1–105), suggesting the antibody may recognize both native and denatured forms of the target.
• For Western blotting, a band at approximately 12 kDa is expected; consider using appropriate protein extraction and lysis conditions to preserve calcium-dependent conformations.
• In immunofluorescence/ICC, note the dual cytoplasmic and nuclear localization; include relevant controls to confirm specific staining patterns.
• This polyclonal antibody is suitable for ELISA; titration and validation in the relevant sample matrix are recommended to optimize performance.
• As with all polyclonal antibodies, lot-to-lot consistency should be verified for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD developers and research institutions by providing a comprehensive range of raw materials, including validated polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents. With this S100A11 polyclonal antibody, researchers studying keratinocyte biology and epidermal differentiation can access a reliable reagent backed by efficient sourcing and supply management. Whether you need standard catalog products or custom rare-target solutions, CamelBio streamlines the path from concept to clinical application.