Anti-S100A11 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P31949

Core Product Specifications and Parameters

Biological Background: S100A11 Function and Localization

• S100A11, also known as calgizzarin, MLN70, or S100-C, is a member of the S100 family of calcium-binding proteins.
• It facilitates the differentiation and cornification of keratinocytes, processes essential for epidermal barrier formation.
• The protein localizes to both the cytoplasm and nucleus, suggesting multiple cellular functions.
• S100A11 contains conserved EF-hand calcium-binding motifs and binds calcium, which may regulate its activity and interactions.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics and direct protein sequencing.
• The protein has a molecular weight of approximately 11.7 kDa and features a disulfide bond, contributing to its three-dimensional structure.

Experimental Guidance and Technical Tips

• For Western blot, given the small size of S100A11 (~12 kDa), consider using a high-percentage acrylamide gel (e.g., 15%) or a gradient gel to improve resolution.
• In immunohistochemistry, antigen retrieval conditions may need optimization; test both citrate (pH 6) and EDTA (pH 9) buffers depending on tissue fixation.
• When performing immunofluorescence, permeabilization with a detergent such as Triton X-100 is typically required for intracellular targets; titrate the antibody to determine the optimal working concentration.
• For ELISA, explore using the antibody as a capture or detection reagent; validate potential cross-reactivity with other S100 family members if multiplexing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with reliable access to IVD raw materials, including validated monoclonal antibodies like this anti-S100A11 antibody, along with bulk ancillary reagents and technical support. Our platform supports keratinocyte biology and calcium-signaling research areas by offering optimized antibody pairs, recombinant proteins, and custom sourcing for rare targets. From concept to clinic, we help streamline your assay development with a comprehensive supply chain.