Anti-S100A10 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P60903

Core Product Specifications and Parameters

Biological Background: S100A10 Function and Localization

• Protein S100-A10 (also known as p11, calpactin I light chain, and cellular ligand of annexin II) is a member of the S100 family of EF-hand calcium-binding proteins.
• S100A10 functions as a regulator of protein phosphorylation by inducing the dimerization of ANXA2/p36; the monomeric form of ANXA2 is the preferred in vitro substrate for tyrosine-specific kinases, suggesting a role in modulating signal transduction.
• The three-dimensional structure of S100A10 has been determined, contributing to understanding its interaction with ANXA2 and other binding partners.
• Post-translational modifications include acetylation, isopeptide bond formation, and ubiquitin-like conjugation, which may influence its stability, localization, or function.
• S100A10 is included in the human reference proteome and has been identified by proteomics studies, confirming its endogenous expression.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein representing the full-length human S100A10 sequence (aa 1–97); thus, the polyclonal antibody may recognize multiple epitopes, including linear and conformational ones.
• For Western blot (WB), S100A10 has a predicted molecular weight of 11 kDa; consider using a 15% SDS-PAGE gel or gradient gel to achieve optimal separation, and ensure appropriate molecular weight markers for small proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate the antibody in known positive tissues or cell lines expressing S100A10; antigen retrieval methods may enhance detection.
• For ELISA, consider pairing with other S100A10 antibodies or using recombinant protein as a standard; confirm specificity with negative controls due to potential cross-reactivity with other S100 family members.
• The antibody is listed as reactive with human, mouse, and rat; however, always verify reactivity in your specific experimental system, as epitope conservation may vary.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted partner for diagnostic manufacturers and research laboratories, CamelBio provides streamlined access to validated IVD raw materials, including polyclonal antibodies like this Anti-S100A10 antibody. Our offerings cover the full development pipeline, from concept to clinic, supporting cell signaling studies with S100A10/ANXA2 interaction assays. Whether you need optimized antibody pairs, bulk ancillary reagents, or rare-target sourcing, CamelBio combines scientific expertise with flexible supply solutions to accelerate your project.