Anti-S100A1 Monoclonal Antibody for WB, IHC-P, ELISA - P23297

Core Product Specifications and Parameters

Biological Background: S100A1 Function and Localization

• S100A1, also known as S-100 protein alpha chain or S100 calcium-binding protein A1, is a small member of the S100 family of EF-hand calcium-binding proteins.
• It acts as a calcium sensor, binding Ca²⁺ to undergo conformational changes that enable interaction with and regulation of downstream target proteins, thereby influencing processes such as Ca²⁺ homeostasis, chondrocyte biology, and cardiomyocyte function (PubMed:12804600, PubMed:23351007, PubMed:22399290). Related references: PMID:12804600 PMID:23351007 PMID:22399290
• In cardiomyocytes, S100A1 coordinates sarcoplasmic reticulum Ca²⁺ cycling and mitochondrial energy production through direct binding to ryanodine receptors (RYR1/RYR2), SERCA2 (ATP2A2), and mitochondrial F1-ATPase, enhancing diastolic relaxation (PubMed:12804600, PubMed:11717446). Related references: PMID:12804600 PMID:11717446
• Subcellularly, S100A1 localizes to the cytoplasm, sarcoplasmic reticulum, and mitochondria, reflecting its multifaceted role in Ca²⁺ handling and bioenergetics.
• Expression is highly enriched in the heart, with lower levels detected in skeletal muscle and brain (PubMed:12804600, PubMed:1384693). Related references: PMID:12804600 PMID:1384693
• Post-translational modifications such as glutathionylation and S-nitrosylation can modulate S100A1 activity in redox-sensitive signaling pathways.

Experimental Guidance and Technical Tips

• For Western blotting, note that S100A1 has a predicted molecular weight of approximately 11 kDa; use a 12–15% SDS-PAGE gel and optimize transfer conditions for low-molecular-weight proteins.
• In IHC-P, validate the antibody using positive control tissues such as cardiac muscle, and consider heat-induced epitope retrieval protocols for formalin-fixed samples.
• When working with calcium-binding proteins like S100A1, EDTA or EGTA in lysis or dilution buffers may alter epitope accessibility; confirm compatibility with your specific assay format.
• For ELISA, pair this monoclonal antibody with a suitable capture/detection reagent and verify performance using recombinant S100A1 protein as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated sourcing partner for IVD manufacturers and research laboratories, offering reliable access to validated antibodies, antigens, and ancillary reagents across diverse disease areas. This recombinant monoclonal antibody against S100A1 supports cardiac biomarker research and calcium signaling studies; CamelBio can supply bulk quantities, customized formulations, and technical guidance to accelerate your assay development. Contact our team to discuss your specific raw material requirements and project goals.