Anti-RYR2 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q92736

Core Product Specifications and Parameters

Biological Background: Ryanodine Receptor 2 (RYR2) Function and Localization

• RYR2 (Ryanodine receptor 2) is a cytosolic calcium-activated calcium channel that mediates the release of Ca2+ from the sarcoplasmic reticulum into the cytosol, playing a key role in triggering cardiac muscle contraction.
• Aberrant RYR2 channel activation can lead to cardiac arrhythmia, highlighting its critical role in maintaining normal heart rhythm.
• In cardiac myocytes, calcium release is triggered by increased cytosolic Ca2+ levels due to activation of the L-type calcium channel CACNA1C.
• The calcium channel activity of RYR2 is modulated by formation of heterotetramers with RYR3, and the protein is essential for cellular calcium ion homeostasis.
• RYR2 is required for embryonic heart development, underscoring its developmental importance.
• The protein is localized to the sarcoplasmic reticulum membrane and is detected in heart muscle (at protein level), as well as in brain (cerebellum and hippocampus) and placenta.
• RYR2 is associated with disease variants linked to cardiomyopathy and arrhythmia, including ARVC2 and ARVD2, as reflected in its alternative names.
• Keywords for RYR2 include calcium channel, calmodulin-binding, cardiomyopathy, disease variant, and sarcoplasmic reticulum, among others.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a cytoplasmic region (amino acids 4868–4967), which may be suitable for detecting total RYR2 protein in western blotting and other applications.
• For immunofluorescence (IF-P) or immunohistochemistry (IHC-P), validate the antibody in your specific tissue type, as cross-reactivity with other ryanodine receptor isoforms (e.g., RYR1, RYR3) should be ruled out.
• Consider using appropriate controls, such as RYR2 knockout cell lines or tissues, to confirm specificity, especially when working with samples expressing multiple ryanodine receptors.
• Due to the large protein size (565 kDa), ensure optimal sample preparation and transfer conditions for western blotting (e.g., low-percentage SDS-PAGE gels, extended transfer times).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For RYR2-related research in cardiac physiology and arrhythmia, CamelBio can support your sourcing needs from concept to clinic. Contact us for tailored solutions and rare target raw-material sourcing.