Anti-RYR2 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q92736

Core Product Specifications and Parameters

Biological Background: RYR2 Function and Localization

• Ryanodine receptor 2 (RYR2) is the major calcium release channel in cardiac muscle sarcoplasmic reticulum, essential for excitation-contraction coupling. It opens upon cytosolic calcium influx via L-type channels, triggering massive calcium release and myocardial contraction.
• RYR2 is a member of the ryanodine receptor family of intracellular ligand-gated ion channels and can form functional heterotetramers with RYR3 to modulate channel activity.
• The protein contains multiple transmembrane segments and is localized exclusively in the sarcoplasmic reticulum membrane.
• Beyond its constitutive role in calcium-induced calcium release, RYR2 is required for cellular calcium ion homeostasis and proper embryonic heart development.
• Aberrant RYR2 activation or mutations are linked to cardiac arrhythmias and cardiomyopathies (e.g., ARVC2, ARVD2, VTSIP), underscoring its disease relevance.
• Tissue specificity includes high expression in heart muscle, with additional detection in brain (cerebellum, hippocampus) and placenta.
• Phosphorylation regulates RYR2 function; the protein is annotated as a phosphoprotein, with potential modulation by kinases in health and disease.

Experimental Guidance and Technical Tips

• Due to its exceptionally large size (~565 kDa), consider using low‑percentage SDS‑PAGE (e.g., 3–5% acrylamide) and prolonged transfer times for effective western blot detection.
• For immunofluorescence, verify expression and proper localization in cardiac or skeletal muscle cell lines; a suitable recombinant protein fragment may serve as a positive control.
• This monoclonal antibody has been tested in multiple applications (WB, IF/ICC, IHC‑P, ELISA) and across human, mouse, and rat species. We recommend independent titration and validation in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with reliable access to this anti‑RYR2 antibody alongside a broad portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Leveraging our expertise in cardiac biomarker sourcing, we support your projects—from rare target procurement to custom reagent development—simplifying your supply chain for faster progress.