Anti-RYR1 Polyclonal Antibody for WB, ELISA - P21817

Core Product Specifications and Parameters

Biological Background: RYR1 Function and Localization

• Cytosolic calcium-activated calcium channel that mediates the release of Ca²⁺ from the sarcoplasmic reticulum into the cytosol, playing a key role in triggering muscle contraction upon T-tubule depolarization. Related references: PMID:11741831 PMID:16163667 PMID:18268335
• Repeated high-level exercise increases RYR1 open probability, leading to Ca²⁺ leakage into the cytoplasm, which may contribute to muscle fatigue and impaired function. Related references: PMID:18268335
• Mediates Ca²⁺ release from intracellular stores in neurons, potentially promoting prolonged calcium signaling in the brain.
• Essential for normal embryonic development of skeletal muscle fibers, heart morphogenesis, skin development, and ossification (By similarity).
• Localized to the sarcoplasmic reticulum membrane in muscle cells.
• Highly expressed in skeletal muscle and specific brain regions (cerebellum and hippocampus).
• Classified as a ligand-gated ion channel with ATP- and calmodulin-binding capabilities; various post-translational modifications including phosphorylation and S-nitrosylation are reported. Mutations are linked to malignant hyperthermia and central core disease.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide within the 800-900 amino acid region of human RYR1. Consider sequence alignment with mouse RYR1 to assess cross-reactivity when designing experiments.
• Validated applications include Western blot (WB) and ELISA. For WB, due to the large molecular weight of RYR1 (~565 kDa), use low-percentage SDS-PAGE gels, extended transfer times, and appropriate high-molecular-weight markers.
• Cross-reactivity with mouse has been confirmed; for other species, verify sequence homology and validate in the relevant sample system before routine use.
• The antibody may be suitable for other applications like immunohistochemistry or immunoprecipitation; however, these require independent optimization and validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers and researchers with seamless access to a broad portfolio of IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and specialized sourcing for rare targets. For projects centered on muscle physiology, calcium signaling, and related pathologies, the Anti-RYR1 Polyclonal Antibody represents a reliable reagent within our comprehensive one-stop offering, streamlining your transition from concept to clinic.