Anti-RXRB Rabbit Polyclonal Antibody for WB, ELISA - P28702

Core Product Specifications and Parameters

Biological Background: Retinoic Acid Receptor RXR-beta Function and Localization

• Retinoic acid receptor RXR-beta, encoded by RXRB, is also known as nuclear receptor subfamily 2 group B member 2, NR2B2, RXRbeta, and retinoid X receptor beta. It is a member of the nuclear receptor family involved in ligand-dependent transcriptional regulation.
• RXR-beta functions as a receptor for retinoic acid, forming heterodimers with retinoic acid receptors (RARs) that bind to retinoic acid response elements (RARE) to modulate gene expression in various biological processes.
• The RAR/RXR heterodimers are key transcriptional regulators that respond to all-trans or 9-cis retinoic acid, playing roles in development, differentiation, and homeostasis.
• The protein localizes to both the nucleus and cytoplasm, consistent with its dynamic role in signal transduction and gene regulation.
• RXR-beta is expressed in aortic endothelial cells (at protein level) and in monocytes, as well as in a variety of tumor cell lines. Related references: PMID:28167758 PMID:26463675
• Structurally, it contains zinc-finger DNA-binding motifs and is subject to alternative splicing and methylation, which may affect its function and stability.
• The calculated molecular weight is approximately 57 kDa (UniProt: 56922 Da), consistent with the reported protein weight.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to amino acids 200-300 of human RXRB (NP_001257330.1). This region may provide specificity for RXRB; however, due to the polyclonal nature, cross-reaction with related isoforms or family members should be considered.
• For Western blotting, the predicted protein weight is 57 kDa. Validate the antibody in human, mouse, and rat samples as cross-reactivity has been indicated, but expression levels may vary across tissues.
• In ELISA applications, optimize coating and detection conditions, as no specific titer or working dilution is supplied. Pair with appropriate secondary antibodies for detection.
• Consider that the protein can localize to both the nucleus and cytoplasm; thus, sample preparation for whole-cell lysates is recommended for comprehensive detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop platform for IVD raw materials, providing diagnostic manufacturers and researchers with reliable access to a wide range of validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our offering includes targets such as RXRB/RXR-beta, supporting investigations into retinoic acid signaling, nuclear receptor biology, and transcriptional regulation. With a seamless supply chain and technical consulting, CamelBio accelerates your development from concept to clinic.