Anti-RXRα Rabbit Polyclonal Antibody for WB, ELISA - P19793

Core Product Specifications and Parameters

Biological Background: RXRα Function and Localization

• RXRα functions as a ligand-dependent transcription factor, binding to retinoic acid response elements (RARE) as homodimers or heterodimers with retinoic acid receptors (RARs) to regulate gene expression. Related references: PMID:10874028, PMID:10195690, PMID:11162439, PMID:11915042
• It is activated by 9-cis retinoic acid; unliganded RXR-RAR heterodimers recruit corepressors leading to chromatin condensation and transcriptional repression, while ligand binding triggers corepressor dissociation and coactivator recruitment. Related references: PMID:1310260, PMID:20215566, PMID:37478846, PMID:9267036
• RXRα serves as a common heterodimeric partner for nuclear receptors like PPARA and RARB, influencing fatty acid oxidation, microRNA regulation, and vascular endothelial responses. Related references: PMID:10195690, PMID:11915042, PMID:28167758, PMID:29021580
• It promotes myelin debris phagocytosis and remyelination by macrophages and attenuates the innate immune response during viral infections by repressing type I IFN genes. Related references: PMID:26463675, PMID:25417649
• The receptor is involved in calcium signaling regulation by repressing ITPR2 gene expression, thereby controlling cellular senescence. Related references: PMID:30216632
• RXRα localizes to the nucleus, cytoplasm, and mitochondrion, and is expressed in liver, kidney, brain, lung fibroblasts, and monocytes.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination; the protein features zinc-finger DNA-binding domains.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100). Consider sequence alignment to confirm epitope conservation across your target species.
• For Western blot, the predicted molecular weight is approximately 51 kDa; note that post-translational modifications may cause slight shifts. Validate antibody specificity with appropriate controls.
• In ELISA applications, optimize coating antigen and antibody concentrations using the relevant sample matrix to ensure linearity and sensitivity.
• Given the expression of RXRα across multiple tissues, include positive and negative controls (e.g., known expressing and knockout cell lysates) to verify specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop partner for diagnostic manufacturers and research labs, offering a wide range of IVD raw materials, technical support, and consulting services from concept to clinic. For RXRα-focused research and assay development, we supply validated polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents. Our sourcing capabilities also extend to rare targets and custom antibody generation, helping streamline your nuclear receptor-related projects.