Anti-RUVBL1/TIP49A/PONTIN Rabbit Monoclonal Antibody for WB, IHC-P, ELISA, ChIP - Q9Y265

Core Product Specifications and Parameters

Biological Background: RUVBL1/TIP49A/PONTIN Function and Localization

• RUVBL1 is a 50 kDa member of the AAA+ ATPase family that functions as a single-stranded DNA-stimulated ATPase and 3'→5' DNA helicase, with hexameric ring formation critical for its enzymatic activity. Related references: PMID:17157868, PMID:33205750
• Serves as a core component of the INO80 and SWR1-like chromatin remodeling complexes, exhibiting nucleosome-stimulated ATPase activity and ATP-dependent nucleosome sliding; also part of the NuA4 histone acetyltransferase complex that acetylates histones H4 and H2A. Related references: PMID:16230350, PMID:21303910, PMID:14966270, PMID:24463511
• Plays an essential role in oncogenic transformation by MYC and modulates transcriptional activation through the LEF1/TCF1-β-catenin pathway. Related references: PMID:10882073, PMID:16014379
• Required for cell proliferation, highlighting its fundamental role in cell cycle progression. Related references: PMID:14506706
• Localizes to multiple subcellular compartments, including the nucleus (nucleoplasm, nuclear matrix), cytoplasm, centrosome, and cell membrane, and associates with dynein axonemal particles.
• Post-translationally modified by acetylation, isopeptide bond formation, and ubiquitin-like conjugation, which may regulate its diverse functions.
• May bind plasminogen at the cell surface and enhance its activation, suggesting a link to extracellular proteolysis. Related references: PMID:11027681
• Ubiquitously expressed, with highest levels in heart, skeletal muscle, and testis.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 350-456), which may provide epitope specificity distal to the conserved ATPase domains, potentially reducing cross-reactivity with closely related family members such as RUVBL2.
• For Western blot, a single band at ~50 kDa is expected; validate in the appropriate cell lysate (e.g., HeLa, HEK293) and optimize blocking and detection conditions.
• In IHC-P, consider heat-induced antigen retrieval (e.g., citrate buffer, pH 6.0) and verify the expected nuclear localization.
• For ChIP, antibody amount and cross-linking conditions need empirical optimization; the antibody may be suitable for enriching genomic regions associated with INO80 or NuA4 complexes.
• Cross-reactivity with mouse and rat allows applications in rodent models, but signal intensity and background should be titrated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and research teams with streamlined access to IVD raw materials, including validated antibodies like this RUVBL1 monoclonal antibody, for chromatin remodeling and oncology studies. Beyond individual reagents, we supply optimized antibody pairs, bulk ancillary reagents, and custom sourcing for challenging targets. Partner with us to accelerate your assay development from concept to clinic.