Anti-RUVBL1/TIP49A/PONTIN Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y265

Core Product Specifications and Parameters

Biological Background: RUVBL1/TIP49A/PONTIN Function and Localization

• Possesses single-stranded DNA-stimulated ATPase and ATP-dependent 3' to 5' DNA helicase activity; hexamerization is critical for ATP hydrolysis and adjacent subunits contribute to the ATPase activity.
  Related references:
  PMID:17157868
  PMID:33205750

• Component of the NuA4 histone acetyltransferase complex, which activates transcription by acetylating histones H4 and H2A, modulating nucleosome-DNA interactions and promoting interaction with transcriptional regulators; the complex also plays a role in DNA repair.
  Related references:
  PMID:14966270

• Part of a SWR1-like complex that mediates removal of histone variant H2A.Z/H2AZ1 from nucleosomes.
  Related references:
  PMID:24463511

• Core component of the chromatin remodeling INO80 complex, which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding.
  Related references:
  PMID:16230350
  PMID:21303910

• Plays an essential role in oncogenic transformation by MYC and modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.
  Related references:
  PMID:10882073
  PMID:16014379

• Essential for cell proliferation.
  Related references:
  PMID:14506706

• May bind plasminogen at the cell surface and enhance plasminogen activation.
  Related references:
  PMID:11027681

• Subcellular localization includes nucleus matrix, nucleoplasm, cytoplasm, membrane, centrosome, and dynein axonemal particles; ubiquitously expressed with high levels in heart, skeletal muscle, and testis.

Experimental Guidance and Technical Tips

• For Western blot (WB), the expected molecular weight is approximately 50 kDa. Optimize blocking and antibody dilution for your specific lysate; validate using known positive controls from human or mouse tissues.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider the diverse subcellular localizations (nuclear, cytoplasmic, membrane). Include appropriate negative controls and validate in relevant tissue sections or cell lines expressing RUVBL1.
• As a polyclonal antibody, it may detect multiple isoforms or post-translationally modified forms. Confirm specificity by siRNA knockdown or comparison with known expression patterns.
• For ELISA, the antibody can be used as a capture or detection reagent; pair it with a matched detection antibody and titrate to determine optimal working concentrations.
• Cross-reactivity is reported with human and mouse, enabling comparative studies; test in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target procurement services to support your entire diagnostic development pipeline. This anti-RUVBL1 polyclonal antibody, targeting a critical chromatin remodeling factor, is ideal for epigenetics and oncology research applications. Contact us to explore custom sourcing solutions and accelerate your product development.