Anti-RUSC1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q9BVN2

Core Product Specifications and Parameters

Biological Background: RUSC1 Function and Localization

• RUSC1 (AP-4 complex accessory subunit RUSC1, also known as Nesca or RUN and SH3 domain-containing protein 1) is a multidomain signaling adapter that associates with the adapter-like complex 4 (AP-4) and may facilitate vesicular trafficking of proteins at the trans-Golgi network.
  Related references:
  PMID:30262884
• It plays a critical role in neuronal differentiation and is involved in the regulation of NGF-dependent neurite outgrowth through signaling pathways that require prolonged activation of MAPK.
  Related references:
  PMID:15024033
• The protein may participate in neuronal vesicular trafficking, particularly involving pre-synaptic membrane proteins, and localizes to early endosomes and the postsynaptic density.
• RUSC1 can regulate the polyubiquitination of IKBKG (NEMO), thereby modulating the NF-kappa-B pathway.
  Related references:
  PMID:19365808
• At the subcellular level, RUSC1 is found in the cytoplasm, nucleus, cytoskeleton, cytoplasmic vesicles, early endosomes, postsynaptic density, and Golgi apparatus.
• The protein is predominantly expressed in brain and contains an SH3 domain, undergoes alternative splicing, and is subject to phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–320, spanning the N-terminal region of human RUSC1; this region may capture multiple isoforms.
• For western blotting, anticipate a band around the predicted 96 kDa, but consider potential post-translational modifications or isoform variants that could shift the apparent mass.
• Given predominant brain expression, enrich or use neural tissue lysates (e.g., human, mouse, or rat brain) to improve detection sensitivity.
• In immunofluorescence/IF-P, optimize permeabilization conditions, as RUSC1 localizes to various intracellular compartments including Golgi, endosomes, and cytoskeleton.
• As a polyclonal antibody, it may exhibit batch-to-batch variability; include appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers in neuroscience and cell biology by supplying high-quality IVD raw materials including validated RUSC1 antibodies. We offer a comprehensive portfolio of polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development and manufacturing needs.