Anti-RUNX3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13761

Core Product Specifications and Parameters

Biological Background: RUNX3 Function and Localization

• Runt-related transcription factor 3 (RUNX3), also known as AML2 or CBFA3, is a member of the RUNX family of transcription factors that play critical roles in cell lineage specification and differentiation.
• RUNX3 forms a heterodimeric complex with core-binding factor beta (CBFB) to constitute the core-binding factor (CBF). The complex recognizes the consensus DNA sequence 5'-TGTGGT-3' (or rarely 5'-TGCGGT-3') via the runt domain, regulating transcription of target genes.
• In response to TGF-beta stimulation, RUNX3 cooperates with ZFHX3 to up-regulate CDKN1A (p21) promoter activity, thereby contributing to growth inhibition. Related references: PMID:20599712
• During cytotoxic T cell development, CBF complexes containing RUNX3 act as transcriptional silencers at the ZBTB7B locus, enabling CD8+ T cell lineage commitment through epigenetic silencing.
• RUNX3 is necessary for the development and survival of parvalbumin-expressing sensory neurons, highlighting its role in neuronal specification.
• The protein is predominantly localized in the nucleus but can also be found in the cytoplasm, consistent with its function as a transcription factor with potential signaling-dependent shuttling.
• Post-translational modifications including phosphorylation and ubiquitination (Ubl conjugation) regulate RUNX3 stability and activity, while alternative splicing may generate multiple isoforms.
• Clinically, RUNX3 is frequently inactivated or misexpressed in gastric cancer and other epithelial cancers, and its tumor-suppressive functions make it a key focus in oncology research.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 286–415 of human RUNX3) corresponds to the C-terminal region. Users should verify that the antibody recognizes the target band at ~44 kDa in WB, although actual migration may shift due to phosphorylation or other modifications.
• For IF/ICC, nuclear and/or cytoplasmic staining is expected. Researchers should validate in their cell system using appropriate controls, as localization may vary with cell type or stimulation.
• This polyclonal antibody may recognize both human and mouse RUNX3, but cross-reactivity should be confirmed if used outside these species.
• In ELISA, consider using the recombinant protein immunogen as a positive control to establish assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research labs with reliable antibodies and ancillary reagents. For RUNX3 research, we can assist in sourcing validated antibody pairs, bulk polyclonal antibodies, and rare target reagents to support projects in oncology and immune cell biology. Reach out to explore tailored procurement solutions for your transcription factor and cancer biomarker development needs.