Anti-RUNX1T1 Polyclonal Antibody for WB, ELISA - Q06455

Core Product Specifications and Parameters

Biological Background: RUNX1T1 Function and Localization

• RUNX1T1, also known as ETO/MTG8/CBFA2T1, is a transcriptional corepressor that facilitates gene silencing via DNA-binding transcription factors and recruitment of histone-modifying enzymes. Related references: PMID:10688654, PMID:12559562, PMID:15203199
• It can repress the expression of MMP7 in a ZBTB33-dependent manner. Related references: PMID:23251453
• RUNX1T1 inhibits transactivation mediated by TCF12. Related references: PMID:16803958
• It acts as a negative regulator of adipogenesis.
• The AML1-MTG8/ETO fusion protein, resulting from the t(8;21) chromosomal rearrangement, is frequently found in acute myeloid leukemia and contributes to leukemogenesis by enhancing hematopoietic stem/progenitor cell self-renewal. Related references: PMID:23812588
• RUNX1T1 is primarily localized to the nucleus.
• It is most abundantly expressed in brain, with lower levels in lung, heart, testis, and ovary.
• The protein contains zinc-finger MYND-type domains and is subject to phosphorylation; it is classified as a DNA-binding proto-oncogene and transcriptional repressor.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment covering amino acids 250–403 of human RUNX1T1 (NP_783552.1), which may recognize both wild-type and certain fusion proteins; validate in the relevant sample system.
• For Western blotting, the predicted molecular weight is approximately 68 kDa, but post-translational modifications may cause band shifts; consider using appropriate controls.
• The reported cross-reactivity with rat suggests potential utility in rodent models, but experimental verification is recommended for each new application.
• ELISA conditions (coating concentration, detection antibody dilution) should be optimized empirically; the antibody may be suitable as a capture or detection reagent depending on the pair.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including this anti-RUNX1T1 polyclonal antibody for transcriptional regulation and leukemia research. Beyond individual antibodies, our portfolio spans validated antibody pairs, recombinant proteins, and ancillary reagents, supported by technical and regulatory expertise to accelerate your assay development from concept to clinic.