Anti-RUFY1 Rabbit Polyclonal Antibody for WB and ELISA - Q96T51

Core Product Specifications and Parameters

Biological Background: RUFY1 Function and Localization

• RUFY1, also known as RABIP4 or ZFYVE12, is a RUN and FYVE domain-containing protein that functions as an activating adapter in endosomal cargo sorting.
• It regulates retrieval of proteins from early/recycling endosomes to the trans-Golgi network through interaction with the dynein-dynactin complex. Related references: PMID:36282215
• Acts as a dual effector of RAB4B and RAB14, mediating a cooperative interaction that facilitates endosomal tethering and fusion. Related references: PMID:20534812
• Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate (PI3P) and participates in early endosomal trafficking. Related references: PMID:14617813
• In oocytes, self-assembles to form endo-lysosomal vesicular assemblies (ELVAs) that sequester ubiquitinated protein aggregates and degrade them upon oocyte maturation (By similarity).
• Localizes predominantly to the membrane of early endosomes.
• Broadly expressed across tissues, with highest levels in lung, testis, kidney, and brain.
• Contains a FYVE zinc-finger domain responsible for PI3P binding; subject to phosphorylation and alternative splicing.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band near 80 kDa based on the calculated molecular weight; validate in relevant human cell lysates (e.g., HeLa, HEK293).
• When performing ELISA, consider using the recombinant immunogen (amino acids 439-708) as a positive control to confirm antibody reactivity.
• Cross-reactivity with mouse and rat RUFY1 is reported; verify specificity in your model system using appropriate controls.
• The immunogen covers the C-terminal region; if your samples express alternative isoforms, assess detection capability by examining sequence conservation in the immunogen region.
• Since this is a polyclonal antibody, batch-to-batch consistency should be verified for quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, offering validated antibody pairs, optimized polyclonal antibodies like this anti-RUFY1 pAb, bulk ancillary reagents, and support for custom rare-target procurement. Whether you are developing assays for endosomal trafficking studies or broader cellular transport research, we provide the materials pipeline to accelerate your diagnostic development.