Anti-RTN4RL1 Rabbit Polyclonal Antibody for WB, ELISA - Q86UN2

Core Product Specifications and Parameters

Biological Background: RTN4RL1 Function and Localization

• RTN4RL1 (Reticulon-4 receptor-like 1), also known as Nogo receptor-like 2 (NGRH2) or NgR3, is a cell surface receptor of the Nogo receptor family.
• Plays a functionally redundant role in postnatal brain development and in regulating axon regeneration in the adult central nervous system; contributes to normal axon migration across the brain midline and formation of the corpus callosum.
• Inhibits neurite outgrowth and axon regeneration by binding neuronal chondroitin sulfate proteoglycans; binds heparin (By similarity).
• Restricts the number of dendritic spines and synapses during brain development; signaling mediates activation of Rho and downstream actin cytoskeleton reorganization (PubMed:22325200).
  Related references: PMID:22325200
• Protects motoneurons against apoptosis, likely mediated by MAG (By similarity).
• Localizes to the cell membrane, membrane rafts, perikaryon, and cell projections; anchored via a GPI anchor.
• Predominantly expressed in brain, with lower levels in kidney, lung, mammary gland, placenta, salivary gland, skeletal muscle, and spleen.
• Contains leucine-rich repeat domains and is a glycoprotein; member of the Nogo receptor family.

Experimental Guidance and Technical Tips

• For Western blotting, use standard HRP-conjugated secondary antibodies and chemiluminescent detection. The predicted molecular weight is ~49 kDa, but glycosylation may cause an upward shift; validate with brain tissue or RTN4RL1-expressing cell lysates.
• In ELISA applications, consider using the recombinant immunogen (aa 220-420) or full-length protein as the coating antigen to optimize binding kinetics.
• Cross-reactivity with mouse and rat is indicated, making the antibody suitable for rodent model studies; however, confirm epitope conservation and specificity in the chosen species.
• Due to sequence homology among Nogo receptor family members (e.g., RTN4R, RTN4RL2), verify specificity in your experimental system using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw material sourcing, offering a broad range of validated antibodies, recombinant proteins, and ancillary reagents to support diagnostic development from concept to clinic. The RTN4RL1 antibody addresses key neuroscience research and neurology biomarker applications, and CamelBio can facilitate customized procurement solutions—including bulk orders and rare target sourcing—to streamline your project timelines.