Anti-RTN4 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q9NQC3

Core Product Specifications and Parameters

Biological Background: RTN4 Function and Localization

• RTN4 is a critical regulator of endoplasmic reticulum (ER) morphogenesis, required for the formation and stabilization of ER tubules. It also influences nuclear envelope expansion, nuclear pore complex formation, and proper localization of inner nuclear membrane proteins. Related references: PMID:24262037 PMID:25612671 PMID:27619977 PMID:26906412
• In the nervous system, RTN4 acts as a developmental neurite growth regulatory factor, inhibiting axon-axon adhesion and growth while facilitating neurite branching. It stabilizes neuronal wiring and restricts plasticity in the adult central nervous system. Related references: PMID:10667797 PMID:11201742
• RTN4 modulates vascular remodeling by promoting endothelial cell migration while inhibiting vascular smooth muscle cell migration. It regulates endothelial sphingolipid biosynthesis and controls vascular function and blood pressure.
• This protein plays a role in immune system regulation, mediating ICAM1-induced transendothelial migration of leukocytes and contributing to TLR9 localization and innate immune responses.
• RTN4 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2 by sequestering them from mitochondria to the endoplasmic reticulum. Related references: PMID:11126360
• Isoforms B and C of RTN4 inhibit BACE1 activity and amyloid precursor protein processing, implicating them in Alzheimer's disease-related pathways. Related references: PMID:16965550
• RTN4 localizes to the endoplasmic reticulum membrane, cell membrane, synapse, and cell junctions, reflecting its diverse functional roles.
• Tissue-specific expression varies among isoforms: isoform A is brain- and testis-specific, isoform B is widely expressed (except liver) with high levels in endothelial and vascular smooth muscle cells, isoform C is found in brain, skeletal muscle, and adipocytes, and isoform D is testis-specific. The protein undergoes post-translational modifications such as phosphorylation and acetylation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 200–300 of human RTN4, a region likely conserved across splice variants and across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, the predicted molecular weight is approximately 130 kDa, but multiple isoforms and post-translational modifications may lead to additional bands; validate with appropriate controls.
• In paraffin-embedded immunofluorescence (IF-P), optimize antigen retrieval and fixation conditions, as performance may vary with tissue type.
• When using ELISA, titrate antibody concentration and consider pairing with a monoclonal antibody for sandwich assays; assay conditions should be empirically determined.
• As a polyclonal antibody, different lots may show slight variability; always validate with your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering a comprehensive portfolio that includes validated antibodies, bulk ancillary reagents, and custom sourcing for rare targets. In the neuroscience and ER biology fields, we support diagnostic and research applications with reliable RTN4-related products. From concept to clinic, our supply chain ensures consistent access to critical raw materials, empowering your assay development.